Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the...

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Published inOncology letters Vol. 9; no. 5; pp. 2361 - 2367
Main Authors NISHIKAWA, SHIMPEI, KONNO, MASAMITSU, HAMABE, ATSUSHI, HASEGAWA, SHINICHIRO, KANO, YOSHIHIRO, FUKUSUMI, TAKAHITO, SATOH, TAROH, TAKIGUCHI, SHUJI, MORI, MASAKI, DOKI, YUICHIRO, ISHII, HIDESHI
Format Journal Article
LanguageEnglish
Published Greece D.A. Spandidos 01.05.2015
Spandidos Publications
Subjects
Antigens
cancer stem cell
CD26
CD44
chemotherapy
Diagnosis
Enzymes
gastric cancer
Health aspects
Identification and classification
Stomach cancer
Tumor markers
tumor-initiating cell
CD26
tumor-initiating cell
cancer stem cell
gastric cancer
chemotherapy
CD44
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Summary:Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti-cell surface marker antibody-based array platform, a flow cytometry-based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26+CD44+ cells (6/6 mice formed tumors), moderately tumorigenic CD26+CD44− cells (5/6 mice formed tumors), and weakly or non-tumorigenic CD26−CD44− cells (2/6 mice formed tumors). Furthermore, exposure to 5-fluorouracil significantly increased the proportion of CD26+ cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.
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ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2015.3063