Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in Brassica rapa L

• Published in: Royal Society open science Vol. 6; no. 2; p. 181727
• Main Authors: Li, Dahui, Wu, Di, Li, Shizhou, Dai, Yu, Cao, Yunpeng
• Format: Journal Article
• Language: English
• Published: England The Royal Society 01.02.2019
• Subjects: Biology (Whole Organism); collinearity; evolution; gene duplication; nadph oxidases; respiratory burst oxidase homologues; respiratory burst oxidase homologues; evolution; NADPH oxidases; collinearity; gene duplication
• ISSN: 2054-5703; 2054-5703
• DOI: 10.1098/rsos.181727

NADPH oxidases (NOXs) have been known as respiratory burst oxidase homologues (RBOHs) in plants. To characterize the evolutionary relationships and functions of RBOHs in , 134 homologues were identified from 13 plant species, including 14 members (namely - ) from . There presented 47 gene-pairs among 14 s and other s, consisting of five pairs within , and 15 pairs between and . Together with phylogenetic analysis, the results suggested that whole-genome duplication might have played an important role in gene expansion, and these duplication events occurred after the divergence of the eudicot and the monocot lineages examined. Furthermore, gene expression of s in both and were assayed via qRT-PCR. An RBOH gene, in , was transformed into wild-type plants. The transgenic lines with the overexpressed level of conferred to be more tolerant to heavy metal lead (0.05 mM) than wild-type plants. Overall, this integrated analysis at genome-wide level has provided some information on the evolutionary relationships among plant-specific NOXs and the coordinated diversification of gene structure and function in .