A Precision Microbiome Approach Using Sucrose for Selective Augmentation of Staphylococcus epidermidis Fermentation against Propionibacterium acnes

• Published in: International journal of molecular sciences Vol. 17; no. 11; p. 1870
• Main Authors: Wang, Yanhan, Kao, Ming-Shan, Yu, Jinghua, Huang, Stephen, Marito, Shinta, Gallo, Richard L, Huang, Chun-Ming
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.11.2016; MDPI
• Subjects: Acne; acne vulgaris; Acne Vulgaris - microbiology; Acne Vulgaris - pathology; Acne Vulgaris - therapy; Animals; Antibiosis; Bacterial Load - drug effects; Biomarkers - metabolism; Chemokine CXCL2 - genetics; Chemokine CXCL2 - metabolism; Coculture Techniques; Dysbiosis - microbiology; Dysbiosis - pathology; Dysbiosis - therapy; Ear - microbiology; Ear - pathology; Female; Fermentation - drug effects; Gene Expression; Glycerol - metabolism; Glycerol - pharmacology; Humans; Mice; Mice, Inbred ICR; microbiome; Microbiota; P. acnes; Probiotics - metabolism; Probiotics - pharmacology; Propionibacterium acnes; Propionibacterium acnes - drug effects; Propionibacterium acnes - growth & development; Propionibacterium acnes - pathogenicity; S. epidermidis; Skin; Skin - drug effects; Skin - microbiology; Skin - pathology; Staphylococcus; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Staphylococcus epidermidis - growth & development; Staphylococcus epidermidis - metabolism; Staphylococcus infections; Sucrose; Sucrose - metabolism; Sucrose - pharmacology; S. epidermidis; skin; acne vulgaris; sucrose; P. acnes; microbiome
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms17111870

Acne dysbiosis happens when there is a microbial imbalance of the over-growth of s ( ) in the acne microbiome. In our previous study, we demonstrated that ( , a probiotic skin bacterium) can exploit glycerol fermentation to produce short-chain fatty acids (SCFAs) which have antimicrobial activities to suppress the growth of . Unlike glycerol, sucrose is chosen here as a selective fermentation initiator (SFI) that can specifically intensify the fermentation activity of , but not . A co-culture of and fermenting in the presence of sucrose significantly led to a reduction in the growth of . The reduction was abolished when was co-cultured with non-fermenting . Results from nuclear magnetic resonance (NMR) analysis revealed four SCFAs (acetic acid, butyric acid, lactic acid, and succinic acid) were detectable in the media of sucrose fermentation. To validate the interference of sucrose fermentation with , mouse ears were injected with both and plus sucrose or phosphate buffered saline (PBS). The level of macrophage-inflammatory protein-2 (MIP-2) and the number of in ears injected with two bacteria plus sucrose were considerably lower than those in ears injected with two bacteria plus PBS. Our results demonstrate a precision microbiome approach by using sucrose as a SFI for , holding future potential as a novel modality to equilibrate dysbiotic acne.