Label-free sensitive detection of influenza virus using PZT discs with a synthetic sialylglycopolymer receptor layer

• Published in: Royal Society open science Vol. 6; no. 9; p. 190255
• Main Authors: Erofeev, Alexander S., Gorelkin, Petr V., Kolesov, Dmitry V., Kiselev, Gleb A., Dubrovin, Evgeniy V., Yaminsky, Igor V.
• Format: Journal Article
• Language: English
• Published: England The Royal Society 01.09.2019
• Subjects: Biochemistry and Biophysics; biosensor; influenza; microelectromechanical systems; sialylglycopolymer; virus detection; influenza; sialylglycopolymer; biosensor; microelectromechanical systems; virus detection
• ISSN: 2054-5703; 2054-5703
• DOI: 10.1098/rsos.190255

We describe rapid, label-free detection of Influenza A viruses using the first radial mode of oscillations of lead zirconate titanate (PZT) piezoelectric discs with a 2 mm radius and 100 µm thickness fabricated from a piezoelectric membrane. The discs are modified with a synthetic sialylglycopolymer receptor layer, and the coated discs are inserted in a flowing virus suspension. Label-free detection of the virus is achieved by monitoring the disc radial mode resonance frequency shift. Piezo transducers with sialylglycopolymer sensor layers exhibited a long lifetime, a high sensitivity and the possibility of regeneration. We demonstrate positive, label-free detection of Influenza A viruses at concentrations below 10 5 virus particles per millilitre. We show that label-free, selective, sensitive detection of influenza viruses by home appliances is possible in principle.