Adhesion of Human B Cells to Germinal Centers in Vitro Involves VLA-4 and INCAM-110

• Published in: Science (American Association for the Advancement of Science) Vol. 249; no. 4972; pp. 1030 - 1033
• Main Authors: Freedman, Arnold S., Munro, J. Michael, Rice, G. Edgar, Bevilacqua, Michael P., Morimoto, Chikao, McIntyre, Bradley W., Rhynhart, Kurt, Pober, Jordan S., Nadler, Lee M.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 31.08.1990; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Antibodies; Antibodies, Monoclonal; Antigens, CD - analysis; B cells; B lymphocytes; B-Lymphocytes - cytology; B-Lymphocytes - immunology; B-Lymphocytes - ultrastructure; Biological and medical sciences; Butchering; Cell Adhesion; Cell Adhesion Molecules - immunology; Cell lines; Cells, Cultured; Follicles; Follicular dendritic cells; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Immunology; Lymphocytes; Lymphoid organs: ontogeny, organization, homing phenomenon; Lymphoid tissue; Molecular aspects; Molecular biology; Molecules; Palatine Tonsil - cytology; Palatine Tonsil - immunology; Physiological aspects; Receptors, Very Late Antigen - immunology; Spleen - immunology; Vascular Cell Adhesion Molecule-1; Human; Immunohistochemistry; Amygdala; Germinative center; Cell adhesion molecule; Cell cell interaction; B-Lymphocyte; Adhesion; In vitro; Cell surface; Antigen; Lymphoid organ; Fluorescence microscopy
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1697696

Human B lymphocytes localize and differentiate within the microenvironment of lymphoid germinal centers. A frozen section binding assay was developed for the identification of those molecules involved in the adhesive interactions between B cells and lymphoid follicles. Activated human B cells and B cell lines were found to selectively adhere to germinal centers. The VLA-4 molecule on the lymphocyte and the adhesion molecule INCAM-110, expressed on follicular dendritic cells, supported this interaction. This cellular interaction model can be used for the study of how B cells differentiate.