Products of a Reciprocal Chromosome Translocation Involving the c-myc Gene in a Murine Plasmacytoma

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 3; pp. 829 - 833
• Main Authors: Stanton, Lawrence W., Yang, Jian-Qing, Eckhardt, Laurel A., Harris, Linda J., Birshtein, Barbara K., Marcu, Kenneth B.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.02.1984; National Acad Sciences
• Subjects: Animals; Base Sequence; Cell Line; Chromosomes; Cloning, Molecular; DNA; DNA Restriction Enzymes; Endonucleases; Exons; Mice; myc genes; Nucleotide sequences; Nucleotides; Oncogenes; Plasmacytoma; Plasmacytoma - genetics; Promoter regions; RNA; Single-Strand Specific DNA and RNA Endonucleases; Translocation, Genetic
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.3.829

The structures of the rearranged c-myc gene products derived from a rcp T(12;15) have been investigated in the MPC-11 (IgG2bκ ) plasmacytoma. The rcp T(12;15) in MPC-11 is a reciprocal exchange between the c-myc gene on chromosome 15 and the immunoglobulin γ 2a switch region (Sγ 2a) on chromosome 12. The c-myc gene is broken within a 5′-nontranslated exon, thereby separating the promoter region of the normal c-myc gene from its protein coding sequences. This reciprocal rearrangement results in the loss of 11 base pairs of c-myc sequence and 300 base pairs of Sγ 2asequence at the point of recombination. Sequences that represent the promoter region of the normal c-myc gene are present in the 5′-myc reciprocal fragment. A comparison of the nucleotide sequences at the recombination site of a number of c-myc rearrangements reveals a common feature that may have mechanistic importance for these translocation events.