Characteristic Differences in Inhibitory Effects of Oligodeoxyribonucleotides from DNA on Human Systemic Lupus Erythematodes (SLE) Sera and Rabbit Anti-DNA Sera

The difference in the antigenic determinant size of DNA for sera from patients with SLE and rabbit anti-DNA sera were investigated. Haptenic inhibition studies were carried out by measuring the inhibition of [3H]DNA-antibody binding by three different types of oligonucleotides which were prepared fr...

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Published inThe Tohoku Journal of Experimental Medicine Vol. 122; no. 2; pp. 161 - 166
Main Authors WAKIZAKA, AKIRA, OKUHARA, EIJI
Format Journal Article
LanguageEnglish
Published Japan Tohoku University Medical Press 01.01.1977
Subjects
Animals
antigenic determination
Binding Sites, Antibody - analysis
DNA
DNA - immunology
Epitopes
Humans
Immune Sera
Lupus Erythematosus, Systemic - immunology
Oligodeoxyribonucleotides - immunology
Oligonucleotides - immunology
Rabbits
Salmon
SLE
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Summary:The difference in the antigenic determinant size of DNA for sera from patients with SLE and rabbit anti-DNA sera were investigated. Haptenic inhibition studies were carried out by measuring the inhibition of [3H]DNA-antibody binding by three different types of oligonucleotides which were prepared from formic acid-diphenylamine digests, hydrazinolyzed digests and pancreatic DNase digests, respectively. Oligonucleotides from DNase I digests showed potent inhibitory activity with both SLE sera and rabbit sera. However, the inhibitory activities of purine and pyrimidine oligonucleotides were more potent for SLE sera than for rabbit anti-DNA sera. The determinant size estimated for rabbit sera was in the range of tetra- to heptanueleotide, while in SLE sera it was in the range of di- and trinucleotide.
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ISSN:0040-8727
1349-3329
DOI:10.1620/tjem.122.161