PHOSPHONOMYCIN : ISOLATION FROM FERMENTATION SOURCES

Phosphonomycin, (-)-cis-1, 2-epoxypropylphosphonic acid, was isolated from fermentation sources as a crystalline calcium salt. Methods for ion-exchange adsorption, ion-exchange chromatography using both anion- and cation-exchange resins, gel-permeation chromatography, adsorption chromatography and s...

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Published inJournal of antibiotics Vol. 23; no. 7; pp. 336 - 347
Main Authors CHAIET, LOUIS, MILLER, THOMAS W., GOEGELMAN, ROBERT T., KEMPF, AUGUST J., WOLF, FRANK J.
Format Journal Article
LanguageEnglish
Published Japan JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 01.01.1970
Subjects
Aluminum
Anti-Bacterial Agents - isolation & purification
Chromatography, Gel
Chromatography, Ion Exchange
Chromatography, Thin Layer
Ethers, Cyclic - isolation & purification
Fermentation
Organophosphonates - isolation & purification
Streptomyces - metabolism
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Summary:Phosphonomycin, (-)-cis-1, 2-epoxypropylphosphonic acid, was isolated from fermentation sources as a crystalline calcium salt. Methods for ion-exchange adsorption, ion-exchange chromatography using both anion- and cation-exchange resins, gel-permeation chromatography, adsorption chromatography and solvent partition were investigated. The overall purification was about 5, 000 fold with a yield of 12 %, Conditins for ion-exchange chromatography were developed based on batch equilibration studies. Thin-layer chromatography was used to develop a chromatographic procedure with alumina. The molecular weight estimated from gel-permeation studies was somewhat higher than the true value. A number of other salts which remained amorphous were also prepared.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.23.336