Facile immobilization of glucose oxidase onto gold nanostars with enhanced binding affinity and optimal function
Gold nanoparticles provide a user-friendly and efficient surface for immobilization of enzymes and proteins. In this paper, we present a novel approach for enzyme bioconjugation to gold nanostars (AuNSs). AuNSs were modified with l-cysteine (Cys) and covalently bound to -hydroxysulfosuccinimide (sul...
Saved in:
Published in | Royal Society open science Vol. 6; no. 5; p. 190205 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
The Royal Society
01.05.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Gold nanoparticles provide a user-friendly and efficient surface for immobilization of enzymes and proteins. In this paper, we present a novel approach for enzyme bioconjugation to gold nanostars (AuNSs). AuNSs were modified with l-cysteine (Cys) and covalently bound to
-hydroxysulfosuccinimide (sulfo-NHS) activated intermediate glucose oxidase (GOx) to fabricate a stable and sensitive AuNSs-Cys-GOx bioconjugate complex. Such a strategy has the potential for increased attachment affinity without protein adsorption onto the AuNSs surface. Good dispersity in buffer suspension was observed, as well as stability in high ionic environments. Using the AuNSs-Cys-GOx bioconjugates showed greater sensitivity in the measuring of low concentrations of glucose based on plasmonic and colorimetric detection. Such a novel approach for enzyme immobilization can lead to AuNSs-Cys-GOx bioconjugate complexes that can be used as catalytic nanodevices in nanobiosensors based on oxidases in biomedical applications. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article has been edited by the Royal Society of Chemistry, including the commissioning, peer review process and editorial aspects up to the point of acceptance. Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.4479839. |
ISSN: | 2054-5703 2054-5703 |
DOI: | 10.1098/rsos.190205 |