Functional diversification of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level
The long-chain (≥C 20 ) polyunsaturated fatty acid biosynthesis capacity of fish varies among species, with trophic level hypothesised as a major factor. The biosynthesis capacity is largely dependent upon the presence of functionally diversified fatty acyl desaturase 2 (Fads2) enzymes, since many t...
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Published in | Scientific reports Vol. 9; no. 1; pp. 11199 - 10 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.08.2019
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | The long-chain (≥C
20
) polyunsaturated fatty acid biosynthesis capacity of fish varies among species, with trophic level hypothesised as a major factor. The biosynthesis capacity is largely dependent upon the presence of functionally diversified fatty acyl desaturase 2 (Fads2) enzymes, since many teleosts have lost the gene encoding a Δ5 desaturase (Fads1). The present study aimed to characterise Fads2 from four teleosts occupying different trophic levels, namely
Sarpa salpa
,
Chelon labrosus
,
Pegusa lascaris
and
Atherina presbyter
, which were selected based on available data on functions of Fads2 from closely related species. Therefore, we had insight into the variability of Fads2 within the same phylogenetic group. Our results showed that Fads2 from
S
.
salpa
and
C
.
labrosus
were both Δ6 desaturases with further Δ8 activity while
P
.
lascaris
and
A
.
presbyter
Fads2 showed Δ4 activity. Fads2 activities of herbivorous
S
.
salpa
are consistent with those reported for carnivorous Sparidae species. The results suggested that trophic level might not directly drive diversification of teleost Fads2 as initially hypothesised, and other factors such as the species’ phylogeny appeared to be more influential. In agreement, Fads2 activities from
P
.
lascaris
and
A
.
presbyter
were similar to their corresponding phylogenetic counterparts
Solea senegalensis
and
Chirostoma estor
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-019-47709-0 |