Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity
Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of met...
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| Published in | Journal of toxicological sciences Vol. 49; no. 2; pp. 49 - 53 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japan
The Japanese Society of Toxicology
01.01.2024
Japan Science and Technology Agency |
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| Online Access | Get full text |
| ISSN | 0388-1350 1880-3989 1880-3989 |
| DOI | 10.2131/jts.49.49 |
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| Abstract | Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results provide a reliable D. melanogaster model system for inhalation toxicity study. |
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| AbstractList | Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results provide a reliable D. melanogaster model system for inhalation toxicity study.Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results provide a reliable D. melanogaster model system for inhalation toxicity study. Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results provide a reliable D. melanogaster model system for inhalation toxicity study. |
| Author | Heo, Yong-Ju Kim, Min-Seok Cho, Yoon Park, Hae-Bin Park, Chul Min |
| Author_xml | – sequence: 1 fullname: Park, Hae-Bin organization: Inhalation Toxicology Research Group, Korea Institute of Toxicology, Republic of Korea – sequence: 1 fullname: Kim, Min-Seok organization: Inhalation Toxicology Research Group, Korea Institute of Toxicology, Republic of Korea – sequence: 1 fullname: Heo, Yong-Ju organization: Inhalation Toxicology Research Group, Korea Institute of Toxicology, Republic of Korea – sequence: 1 fullname: Park, Chul Min organization: Division of Practical Research, Honam National Institute of Biological Resources, Republic of Korea – sequence: 1 fullname: Cho, Yoon organization: Inhalation Toxicology Research Group, Korea Institute of Toxicology, Republic of Korea |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38296528$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1534/genetics.117.300167 10.1038/s41598-022-27284-7 10.3346/jkms.2022.37.e101 10.5487/TR.2019.35.2.103 10.1038/s42003-021-02847-y 10.3390/biom13020378 10.1089/aivt.2018.0004 |
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| References | Brown, J.B., Langley, S.A., Snijders, A.M., Wan, K.H., Morris, S.N., Booth, B.W., Fisher, W.W., Hammonds, A.S., Park, S., Weiszmann, R., Yu, C., Kirwan, J.A., Weber, R.J., Viant, M.R., Mao, J.H. and Celniker, S.E. (2021): An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila. Commun. Biol., 4, 1324. El Kholy, S. and Al Naggar, Y. (2023): Exposure to polystyrene microplastic beads causes sex-specific toxic effects in the model insect Drosophila melanogaster. Sci. Rep., 13, 204. Kim, J., Park, S., Zoh, K.E., Park, J., Choi, S., Hwang, S.H., Lee, S.Y. and Park, D.U. (2022): Review of Inhalation Health Risks Involving Chloromethylisothiazolinone (CMIT) and Methylisothiazolinone (MIT) Used as Disinfectants in Household Humidifiers. J. Korean Med. Sci., 37, e101. Brischigliaro, M., Fernandez-Vizarra, E. and Viscomi, C. (2023): Mitochondrial Neurodegeneration: Lessons from Drosophila melanogaster Models. Biomolecules, 13, 378. National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. (1999): Monoclonal Antibody Production. National Academies Press (US), Washington (DC). Hayashi, S. and Kondo, T. (2018): Development and Function of the Drosophila Tracheal System. Genetics, 209, 367-380. Kim, M.K., Kim, K.B., Lee, J.Y., Kwack, S.J., Kwon, Y.C., Kang, J.S., Kim, H.S. and Lee, B.M. (2019): Risk Assessment of 5-Chloro-2-Methylisothiazol-3(2H)-One/2-Methylisothiazol-3(2H)-One (CMIT/MIT) Used as a Preservative in Cosmetics. Toxicol. Res., 35, 103-117. Jackson, G.R. Jr., Maione, A.G., Klausner, M. and Hayden, P.J. (2018): Prevalidation of an Acute Inhalation Toxicity Test Using the EpiAirway In Vitro Human Airway Model. Appl. In Vitro Toxicol., 4, 149-158. 1 2 4 5 6 7 8 S. Hayashi (3) 2018 |
| References_xml | – reference: El Kholy, S. and Al Naggar, Y. (2023): Exposure to polystyrene microplastic beads causes sex-specific toxic effects in the model insect Drosophila melanogaster. Sci. Rep., 13, 204. – reference: Hayashi, S. and Kondo, T. (2018): Development and Function of the Drosophila Tracheal System. Genetics, 209, 367-380. – reference: Brischigliaro, M., Fernandez-Vizarra, E. and Viscomi, C. (2023): Mitochondrial Neurodegeneration: Lessons from Drosophila melanogaster Models. Biomolecules, 13, 378. – reference: Kim, J., Park, S., Zoh, K.E., Park, J., Choi, S., Hwang, S.H., Lee, S.Y. and Park, D.U. (2022): Review of Inhalation Health Risks Involving Chloromethylisothiazolinone (CMIT) and Methylisothiazolinone (MIT) Used as Disinfectants in Household Humidifiers. J. Korean Med. Sci., 37, e101. – reference: Kim, M.K., Kim, K.B., Lee, J.Y., Kwack, S.J., Kwon, Y.C., Kang, J.S., Kim, H.S. and Lee, B.M. (2019): Risk Assessment of 5-Chloro-2-Methylisothiazol-3(2H)-One/2-Methylisothiazol-3(2H)-One (CMIT/MIT) Used as a Preservative in Cosmetics. Toxicol. Res., 35, 103-117. – reference: National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. (1999): Monoclonal Antibody Production. National Academies Press (US), Washington (DC). – reference: Brown, J.B., Langley, S.A., Snijders, A.M., Wan, K.H., Morris, S.N., Booth, B.W., Fisher, W.W., Hammonds, A.S., Park, S., Weiszmann, R., Yu, C., Kirwan, J.A., Weber, R.J., Viant, M.R., Mao, J.H. and Celniker, S.E. (2021): An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila. Commun. Biol., 4, 1324. – reference: Jackson, G.R. Jr., Maione, A.G., Klausner, M. and Hayden, P.J. (2018): Prevalidation of an Acute Inhalation Toxicity Test Using the EpiAirway In Vitro Human Airway Model. Appl. In Vitro Toxicol., 4, 149-158. – start-page: 367 issn: 0016-6731 year: 2018 ident: 3 publication-title: Genetics doi: 10.1534/genetics.117.300167 – ident: 5 doi: 10.1038/s41598-022-27284-7 – ident: 6 doi: 10.3346/jkms.2022.37.e101 – ident: 7 doi: 10.5487/TR.2019.35.2.103 – ident: 2 doi: 10.1038/s42003-021-02847-y – ident: 1 doi: 10.3390/biom13020378 – ident: 4 doi: 10.1089/aivt.2018.0004 – ident: 8 |
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| SubjectTerms | Acute inhalation toxicity Alternative animal model Animal models Animals Diameters Drosophila melanogaster Drosophila melanogaster - genetics Environmental conditions Exposure Fruit flies Genetic analysis Inhalation Inhalation Exposure - adverse effects Life cycles Models, Animal Respiration Sensitivity Survival Thiazoles Toxicity |
| Title | Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity |
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