Drosophila melanogaster as potential alternative animal model for evaluating acute inhalation toxicity

• Published in: Journal of toxicological sciences Vol. 49; no. 2; pp. 49 - 53
• Main Authors: Park, Hae-Bin, Kim, Min-Seok, Heo, Yong-Ju, Park, Chul Min, Cho, Yoon
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society of Toxicology 01.01.2024; Japan Science and Technology Agency
• Subjects: Acute inhalation toxicity; Alternative animal model; Animal models; Animals; Diameters; Drosophila melanogaster; Drosophila melanogaster - genetics; Environmental conditions; Exposure; Fruit flies; Genetic analysis; Inhalation; Inhalation Exposure - adverse effects; Life cycles; Models, Animal; Respiration; Sensitivity; Survival; Thiazoles; Toxicity; Acute inhalation toxicity; Drosophila melanogaster; Alternative animal model
• ISSN: 0388-1350; 1880-3989; 1880-3989
• DOI: 10.2131/jts.49.49

Drosophila melanogaster (D. melanogaster) is a promising model biological system. It has a short life cycle and can provide a substantial number of specimens suitable for comprehensive genetic and molecular analyses in a short time. In this study, we investigated the acute inhalation toxicity of methylisothiazolinone (MIT) and chloromethylisothiazolinone (CMIT) in a D. melanogaster model. During exposure, environmental conditions, mass median aerodynamic and geometric standard diameters were measured. After inhalation exposure, the survival rate, climbing ability, and bang sensitivity were measured on days 1, 2, and 7. Notably, the survival rate of flies decreased in an exposure concentration-dependent manner. Climbing ability and bang sensitivity were also altered in the MIT/CMIT group, compared with the negative control group. Overall, these results provide a reliable D. melanogaster model system for inhalation toxicity study.