Large-scale acoustic-driven neuronal patterning and directed outgrowth

• Published in: Scientific reports Vol. 10; no. 1; p. 4932
• Main Authors: Cohen, Sharon, Sazan, Haim, Kenigsberg, Avraham, Schori, Hadas, Piperno, Silvia, Shpaisman, Hagay, Shefi, Orit
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.03.2020; Nature Publishing Group
• Subjects: 13/107; 631/1647/2204; 692/617; Acoustics; Animals; Cell Culture Techniques; Cells, Cultured; Dorsal root ganglia; Electrophysiological Phenomena; Ganglia, Spinal - cytology; Humanities and Social Sciences; Hydrogels; Mimicry; multidisciplinary; Neurites - physiology; Neurons - physiology; Pattern formation; PC12 Cells; Pheochromocytoma cells; Rats; Science; Science (multidisciplinary); Sound; Tissue Engineering
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-60748-2

Acoustic manipulation is an emerging non-invasive method enabling precise spatial control of cells in their native environment. Applying this method for organizing neurons is invaluable for neural tissue engineering applications. Here, we used surface and bulk standing acoustic waves for large-scale patterning of Dorsal Root Ganglia neurons and PC12 cells forming neuronal cluster networks, organized biomimetically. We showed that by changing parameters such as voltage intensity or cell concentration we were able to affect cluster properties. We examined the effects of acoustic arrangement on cells atop 3D hydrogels for up to 6 days and showed that assembled cells spontaneously grew branches in a directed manner towards adjacent clusters, infiltrating the matrix. These findings have great relevance for tissue engineering applications as well as for mimicking architectures and properties of native tissues.