Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana

As obligate intracellular phytopathogens, plant viruses must take advantage of hosts plasmodesmata and phloem vasculature for their local and long-distance transports to establish systemic infection in plants. In contrast to well-studied virus local transports, molecular mechanisms and related host...

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Published inScientific reports Vol. 9; no. 1; p. 3187
Main Authors Zhang, Zhen, Tong, Xin, Liu, Song-Yu, Chai, Long-Xiang, Zhu, Fei-Fan, Zhang, Xiao-Peng, Zou, Jing-Ze, Wang, Xian-Bing
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 28.02.2019
Nature Publishing Group
Subjects
13/1
13/44
38/22
38/23
38/77
42/41
45
631/449/2169/2674
631/449/2661/2666
Arabidopsis thaliana
Disseminated infection
Genetic analysis
Genetic screening
Green fluorescent protein
Humanities and Social Sciences
Infections
Molecular modelling
multidisciplinary
Plant species
Plant virus diseases
Plant viruses
Plasmodesmata
Proteins
Rosette
Science
Science (multidisciplinary)
Systemic diseases
Viruses
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Summary:As obligate intracellular phytopathogens, plant viruses must take advantage of hosts plasmodesmata and phloem vasculature for their local and long-distance transports to establish systemic infection in plants. In contrast to well-studied virus local transports, molecular mechanisms and related host genes governing virus systemic trafficking are far from being understood. Here, we performed a forward genetic screening to identify Arabidopsis thaliana mutants with enhanced susceptibility to a 2b-deleted mutant of cucumber mosaic virus (CMV-2aT∆2b). We found that an uncharacterized Piezo protein (AtPiezo), an ortholog of animal Piezo proteins with mechanosensitive (MS) cation channel activities, was required for inhibiting systemic infection of CMV-2aT∆2b and turnip mosaic virus tagged a green fluorescent protein (GFP) (TuMV-GFP). AtPiezo is induced by virus infection, especially in the petioles of rosette leaves. Thus, we for the first time demonstrate the biological function of Piezo proteins in plants, which might represent a common antiviral strategy because many monocot and dicot plant species have a single Piezo ortholog.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-39436-3