Strain-specific pathogenicity and subversion of phenoloxidase activity in the mosquito Aedes aegypti by members of the fungal entomopathogenic genus Isaria
Development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and Zika. Fungal-based biopesticides are promising alternatives to synthetic...
Saved in:
Published in | Scientific reports Vol. 8; no. 1; pp. 9896 - 12 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
02.07.2018
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and Zika. Fungal-based biopesticides are promising alternatives to synthetic insecticides because they are ecofriendly and are highly effective at infecting insects through contact. This study evaluated the susceptibility of the yellow fever mosquito
Ae
.
aegypti
to a range of entomopathogenic fungal strains from the genus
Isaria
. We observed a diverse variation in the virulence of the
Isaria
strains tested, with two strains showing high pathogenicity towards adult mosquitoes. Mosquito susceptibility to fungal infection was further corroborated through the molecular quantification of fungal loads and the transcript evaluation of a fungal-specific pathogen recognition molecule in the mosquito body. Moreover, quantitative analysis of transcript abundance coupled with enzymatic assays revealed strain-specific subversion of the melanization cascade, an important immune response component. Our study contributes critical insights for a better understanding of fungal-mosquito interactions. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-018-28210-6 |