Standardization procedure for flow cytometry data harmonization in prospective multicenter studies

One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different in...

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Published inScientific reports Vol. 10; no. 1; p. 11567
Main Authors Le Lann, Lucas, Jouve, Pierre-Emmanuel, Alarcón-Riquelme, Marta, Jamin, Christophe, Pers, Jacques-Olivier
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 14.07.2020
Nature Publishing Group
Subjects
631/114/1314
631/1647/794
Biomarkers
Clinical trials
Flow cytometry
Flow Cytometry - standards
Hematology
Human health and pathology
Humanities and Social Sciences
Humans
Immunology
Leukocytes
Life Sciences
multidisciplinary
Prospective Studies
Reference Standards
Science
Science (multidisciplinary)
Standardization
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Summary:One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects.
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PMCID: PMC7360585
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-68468-3