Shelf Life Evaluation of Clinical Grade Chondrogenic Induced Aged Adult Stem Cells for Cartilage Regeneration

• Published in: Scientific reports Vol. 8; no. 1; pp. 4345 - 11
• Main Authors: Ude, C. C., Seet, W. T., Sharen Aini, S., Aminuddin, B. S., Ruszymah, B. H. I.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.03.2018; Nature Publishing Group
• Subjects: 13/100; 13/107; 13/31; 14/19; 14/35; 14/63; 38/39; 38/77; 42/100; 631/61/2035; 692/308/2171; 692/4023/1671/1354; Bone marrow; Cartilage; Cell proliferation; Embryos; Fibroblast growth factor 2; Humanities and Social Sciences; multidisciplinary; Regeneration; Science; Science (multidisciplinary); Shelf life; Stem cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-22748-1

The study objectives include, enhancing the proliferations of aged bone marrow stem cells (BMSCs) and adipose stem cells (ADSCs); and evaluating the shelf lives of clinical grade chondrogenically induced cells from both samples. ADSCs and BMSCs from 56 patients (76 ± 8 yrs) were proliferated using basal medium (FD) and at (5, 10, 15, 20 and 25) ng/ml of basal fibroblast growth factor (bFGF). They were induced to chondrogenic lineage and stored for more than 120 hrs in FD, serum, Dulbecco’s phosphate buffered saline (DPBS) and saline at 4 °C. In FD, cells stagnated and BMSCs’ population doubling time (PDT) was 137 ± 30 hrs, while ADSCs’ was 129.7 ± 40 hrs. bFGF caused PDT’s decrease to 24.5 ± 5.8 hrs in BMSCs and 22.0 ± 6.5 hrs in ADSCs (p = 0.0001). Both cells were positive to stem cell markers before inductions and thereafter, expressed significantly high chondrogenic genes (p = 0.0001). On shelf life, both cells maintained viabilities and counts above 70% in FD and serum after 120 hrs. BMSCs’ viabilities in DPBS fell below 70% after 96 hrs and saline after 72 hrs. ADSCs’ viability fell below 70% in DPBS after 24 hrs and saline within 24 hrs. Concentrations between 20 ng/ml bFGF is ideal for aged adult cells’ proliferation and delivery time of induced BMSCs and ADSCs can be 120 hrs in 4 °C serum.