Molecular mechanism of lateral bud differentiation of Pinus massoniana based on high-throughput sequencing

• Published in: Scientific reports Vol. 11; no. 1; p. 9033
• Main Authors: Chen, Hu, Tan, Jianhui, Liang, Xingxing, Tang, Shengsen, Jia, Jie, Yang, Zhangqi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.04.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/449/1659; 631/449/2491; 631/449/2653; 631/449/2675; 631/449/447; Abscisic acid; Apical dominance; Autosomal dominant inheritance; Breeding; Buds; Cytokinins; Gene expression; Gene set enrichment analysis; Gibberellins; Humanities and Social Sciences; Indoleacetic acid; Jasmonic acid; Metabolism; multidisciplinary; Next-generation sequencing; Phytohormones; Pinus massoniana; Science; Science (multidisciplinary); Seedlings; Transcription factors; Transcriptomes; Zeatin
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-87787-7

Knot-free timber cultivation is an important goal of forest breeding, and lateral shoots affect yield and stem shape of tree. The purpose of this study was to analyze the molecular mechanism of lateral bud development by removing the apical dominance of Pinus massoniana young seedlings through transcriptome sequencing and identify key genes involved in lateral bud development. We analyzed hormone contents and transcriptome data for removal of apical dominant of lateral buds as well as apical and lateral buds of normal development ones. Data were analyzed using an comprehensive approach of pathway- and gene-set enrichment analysis, Mapman visualization tool, and gene expression analysis. Our results showed that the contents of auxin (IAA), Zea and strigolactone (SL) in lateral buds significantly increased after removal of apical dominance, while abscisic acid (ABA) decreased. Gibberellin (GA) metabolism, cytokinin (CK), jasmonic acid, zeatin pathway-related genes positively regulated lateral bud development, ABA metabolism-related genes basically negatively regulated lateral bud differentiation, auxin, ethylene, SLs were positive and negative regulation, while only A small number of genes of SA and BRASSINOSTEROID, such as TGA and TCH4 , were involved in lateral bud development. In addition, it was speculated that transcription factors such as WRKY, TCP, MYB, HSP, AuxIAA, and AP2 played important roles in the development of lateral buds. In summary, our results provided a better understanding of lateral bud differentiation and lateral shoot formation of P. massoniana from transcriptome level. It provided a basis for molecular characteristics of side branch formation of other timber forests, and contributed to knot-free breeding of forest trees.