Differences in developing intestinal microbiota between allergic and non-allergic infants: A pilot study in Japan

• Published in: Bioscience, biotechnology, and biochemistry Vol. 71; no. 9; pp. 2338 - 2342
• Main Authors: Songjinda, P.(Kyushu Univ., Fukuoka (Japan)), Nakayama, J, Tateyama, A, Tanaka, S, Tsubouchi, M, Kiyohara, C, Shirakawa, T, Sonomoto, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2007; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: allergy; Bacteroidaceae; bacteroides; BEBES; Biological and medical sciences; Clostridium perfringens; ENFANT EN BAS AGE; Enterobacteriaceae; feces; FLORA MICROBIANA; FLORE MICROBIENNE; Fundamental and applied biological sciences. Psychology; gastrointestinal (GI)-tract microbiota; GENERO HUMANO; GENRE HUMAIN; Humans; HYPERSENSITIVITY; Hypersensitivity - microbiology; Infant; INFANTS; INTESTIN; INTESTINES; Intestines - microbiology; INTESTINOS; JAPAN; JAPON; Lactobacillus; MANKIND; MICROBIAL FLORA; MONITORING; Pilot Projects; REACCIONES ALERGICAS; REACTION ALLERGIQUE; RNA, Ribosomal, 16S - genetics; SURVEILLANCE; VIGILANCIA; Asia; Japan; Human; Allergy; Gut; Bacteria; Microflora; Infant; Feces; Digestive tract; gastrointestinal (GI)-tract microbiota; Bacteroidaceae; Bacteroides
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70154

The bacterial compositions of feces were monitored in the first 2 months for 15 infants born in Japan, including eight subjects who developed allergy by the age of 2 years. Primer sets targeting six predominant bacterial groups in the infant intestine, Bacteroidaceae, Enterobacteriaceae, bifidobacteria, enterococci, lactobacilli, and the Clostridium perfringens group, were used for real-time PCR to quantitate each population in the feces. The population of Bacteroidaceae was significantly higher in the allergic group at the ages of 1 month (P=0.03) and 2 months (P=0.05) than in the non-allergic group, while no statistically significant difference was observed for the other bacterial populations.