dual gene‐silencing vector system for monocot and dicot plants

• Published in: Plant biotechnology journal Vol. 12; no. 3; pp. 330 - 343
• Main Authors: Liou, Ming‐Ru, Huang, Ying‐Wen, Hu, Chung‐Chi, Lin, Na‐Sheng, Hsu, Yau‐Heiu
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.04.2014
• Subjects: Bamboo; Bamboo mosaic virus; Brachypodium; Brachypodium - genetics; Brachypodium distachyon; Cloning; DNA methylation; DNA-directed RNA polymerase; Fluorescence; Gene Silencing; Genes; Genetic Vectors; Genetic Vectors - genetics; genetics; Genomics; Green fluorescent protein; Green Fluorescent Proteins; Hsp70 protein; Hsp90 protein; Mathematical analysis; Nicotiana - genetics; Nicotiana benthamiana; plant development; Plant diseases; Plant Leaves; Plant Leaves - genetics; Plant Proteins; Plant Proteins - genetics; Plant viruses; Plants (organisms); Plants, Genetically Modified; Potexvirus; Potexvirus - genetics; Proteins; Ribonucleic acid; Ribonucleic acids; RNA; RNA polymerase; RNA, Satellite; RNA, Satellite - genetics; RNA, Viral; RNA, Viral - genetics; RNA-Dependent RNA Polymerase; RNA-Dependent RNA Polymerase - genetics; RNA‐dependant RNA polymerase 6; Satellite RNA; satellite RNA‐based vector; Satellites; Seedlings; Seedlings - genetics; Silence; Species Specificity; Tobacco; Transgenes; Transgenic; Transgenic plants; Vectors (mathematics); VIGS vector; Virology; Virulence; Viruses; virus‐induced gene silencing; United States > US; satellite RNA; satellite RNA-based vector; virus-induced gene silencing; Bamboo mosaic virus; RNA-dependant RNA polymerase 6; VIGS vector
• ISSN: 1467-7644; 1467-7652; 1467-7652
• DOI: 10.1111/pbi.12140

Plant virus‐based gene‐silencing vectors have been extensively and successfully used to elucidate functional genomics in plants. However, only limited virus‐induced gene‐silencing (VIGS) vectors can be used in both monocot and dicot plants. Here, we established a dual gene‐silencing vector system based on Bamboo mosaic virus (BaMV) and its satellite RNA (satBaMV). Both BaMV and satBaMV vectors could effectively silence endogenous genes in Nicotiana benthamiana and Brachypodium distachyon. The satBaMV vector could also silence the green fluorescent protein (GFP) transgene in GFP transgenic N. benthamiana. GFP transgenic plants co‐agro‐inoculated with BaMV and satBaMV vectors carrying sulphur and GFP genes, respectively, could simultaneously silence both genes. Moreover, the silenced plants could still survive with the silencing of genes essential for plant development such as heat‐shock protein 90 (Hsp90) and Hsp70. In addition, the satBaMV‐ but not BaMV‐based vector could enhance gene‐silencing efficiency in newly emerging leaves of N. benthamiana deficient in RNA‐dependant RNA polymerase 6. The dual gene‐silencing vector system of BaMV and satBaMV provides a novel tool for comparative functional studies in monocot and dicot plants.