Yield and kinetic constants estimation in the production of hydroxy fatty acids from oleic acid in a bioreactor by Pseudomonas aeruginosa 42A2

• Published in: Applied microbiology and biotechnology Vol. 98; no. 23; pp. 9609 - 9621
• Main Authors: Martin-Arjol, Ignacio, Llacuna, Joan Llorens, Manresa, Ángeles
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2014; Springer; Springer Nature B.V
• Subjects: Air flow; Analysis; Bacteria; Biomedical and Life Sciences; Bioreactors; Bioreactors - microbiology; Biotechnological Products and Process Engineering; Biotechnology; Biotransformation; Chromatography; Constants; Fatty acids; Fatty Acids - analysis; Foams; Genetic aspects; Life Sciences; Mathematical models; Membrane reactors; Microbial Genetics and Genomics; Microbiology; Models, Statistical; Monounsaturated fatty acids; Oleic acid; Oleic Acid - metabolism; Physiological aspects; Production processes; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Quantitative analysis; Reaction kinetics; Simulation; Software; Studies; Spain; Non-dispersive aeration system; Bioreactor; Simulation; Hydroxy fatty acids; Kinetic constants; 42A2
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-014-5996-9

We modelled the production of hydroxy fatty acids from oleic acid by Pseudomonas aeruginosa 42A2 in a bioreactor with a non-dispersive aeration system. First, we designed an adapted wetted-wall gas-absorption column, offering a k L a value of 39.9 h −1 , to enhance oxygen absorption in the culture media and prevent foam formation. Then, we analysed different kinetic models to simulate the yield coefficients and the kinetic constants in this bacterial transformation. Monod model fitting ( μ max1  = 0.51 h −1 , K S1  = 1.60 C-mol l −1 , μ max2  = 0.12 h −1 , K S2  = 0.035 C-mol l −1 , and k 2  = 0.033 h −1 ) showed a good accuracy with the experimental data sets and was chosen for its simplicity. Lastly, mass balances were carried out to establish the stoichiometry of this biotransformation with the following yield coefficients, Υ X/OA , Υ X/(10 S )-HPOME and Υ (10 S )-HPOME/(7 S 10 S )-HPOME of 0.172, 0.347 and 2.388 C-mol C-mol −1 , respectively.