Bone Morphogenetic Protein 15 (BMP-15) Improves In Vitro Mouse Folliculogenesis

Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle sur...

Full description

Saved in:
Bibliographic Details
Published inAnimals (Basel) Vol. 13; no. 6; p. 980
Main Authors Jitjumnong, Jakree, Tang, Pin-Chi
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 08.03.2023
MDPI
Subjects
alginate encapsulation
alginates
bone morphogenetic protein 15
Bone morphogenetic proteins
Cancer therapies
estradiol
Fertility
follicular development
Gene expression
glutathione
Growth factors
Hydrogels
in vitro fertilization
Infertility
metaphase
mice
oocytes
ovarian follicles
Ovaries
Oxidative stress
preantral follicle
reactive oxygen species
secretion
survival rate
Transforming growth factors
Taiwan
Minneapolis Minnesota
New York
United States > US
alginate encapsulation
glutathione
progesterone
reactive oxygen species
in vitro fertilization
preantral follicle
bone morphogenetic protein 15
estradiol
Online AccessGet full text

Cover

Abstract Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
AbstractList Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
Multilayered secondary follicles were encapsulated and cultured for 12 days in a 3D culture system by using alginate supplemented with bone morphogenetic protein 15 (BMP-15). Alginate is used in in vitro culture systems to mimic the natural ovarian environment by maintaining the 3D follicular architecture, cell-cell interactions, and paracrine signals underlying direct follicle development. Typically employed for follicle culture, alginate prepared at a concentration of 0.5% w/v led to considerable developmental competence and steroidogenesis. BMP-15 is a member of the superfamily of transforming growth factor-beta (TGF-β), a protein that is necessary for ovarian follicular growth. This protein functions primarily by attaching to its receptor on the surface of granulosa cells, which are also essential for the development of ovarian follicles. Regarding observations of follicle growth, in vitro culture using 0.5% alginate supplemented with 15 ng/mL BMP-15 can promote the production of mature oocytes and increase steroidogenesis. However, mature oocytes retrieved in vitro were subject to hypoxia conditions. Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
Multilayered secondary follicles were encapsulated and cultured for 12 days in a 3D culture system by using alginate supplemented with bone morphogenetic protein 15 (BMP-15). Alginate is used in in vitro culture systems to mimic the natural ovarian environment by maintaining the 3D follicular architecture, cell-cell interactions, and paracrine signals underlying direct follicle development. Typically employed for follicle culture, alginate prepared at a concentration of 0.5% w/v led to considerable developmental competence and steroidogenesis. BMP-15 is a member of the superfamily of transforming growth factor-beta (TGF-β), a protein that is necessary for ovarian follicular growth. This protein functions primarily by attaching to its receptor on the surface of granulosa cells, which are also essential for the development of ovarian follicles. Regarding observations of follicle growth, in vitro culture using 0.5% alginate supplemented with 15 ng/mL BMP-15 can promote the production of mature oocytes and increase steroidogenesis. However, mature oocytes retrieved in vitro were subject to hypoxia conditions.
Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
Simple SummaryMultilayered secondary follicles were encapsulated and cultured for 12 days in a 3D culture system by using alginate supplemented with bone morphogenetic protein 15 (BMP-15). Alginate is used in in vitro culture systems to mimic the natural ovarian environment by maintaining the 3D follicular architecture, cell–cell interactions, and paracrine signals underlying direct follicle development. Typically employed for follicle culture, alginate prepared at a concentration of 0.5% w/v led to considerable developmental competence and steroidogenesis. BMP-15 is a member of the superfamily of transforming growth factor-beta (TGF-β), a protein that is necessary for ovarian follicular growth. This protein functions primarily by attaching to its receptor on the surface of granulosa cells, which are also essential for the development of ovarian follicles. Regarding observations of follicle growth, in vitro culture using 0.5% alginate supplemented with 15 ng/mL BMP-15 can promote the production of mature oocytes and increase steroidogenesis. However, mature oocytes retrieved in vitro were subject to hypoxia conditions.AbstractMultilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
Audience Academic
Author Jitjumnong, Jakree
Tang, Pin-Chi
AuthorAffiliation 1 Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan
2 The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan
AuthorAffiliation_xml – name: 1 Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan
– name: 2 The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan
Author_xml – sequence: 1
  givenname: Jakree
  orcidid: 0000-0002-2538-4528
  surname: Jitjumnong
  fullname: Jitjumnong, Jakree
– sequence: 2
  givenname: Pin-Chi
  orcidid: 0000-0002-0214-6754
  surname: Tang
  fullname: Tang, Pin-Chi
BackLink https://www.ncbi.nlm.nih.gov/pubmed/36978521$$D View this record in MEDLINE/PubMed
BookMark eNqFks9rFDEUxwep2Fp78i4DXioyNclsfsxJ2mJ1oaU9qNeQZN5ss8wmazJT8L_3TbfV3VIwOSQkn-83eV_e62IvxABF8ZaSk7puyCcTPK2JII0iL4oDRqSomKB8b2u_XxzlvCQ4JK8pp6-K_Vo0UnFGD4rrM_Qrr2Ja38YFBBi8K29SHMCHkvLy-OzqpqL8QzlfrVO8g1zOQ_nTDymiZsxQXsS-927s78XZ5zfFy870GY4e1sPix8WX7-ffqsvrr_Pz08vKcSmGqm2AgBKssYbVneyI6oQSppGU2ZqAm1Hcy1pwaXjNmZTSulYyhBWAlF19WMw3vm00S71OfmXSbx2N1_cHMS20SVhMD1qITlms1loqZ0IIS1ulrFBAjLEgJHp93nitR7uC1kEYkul3THdvgr_Vi3inKSGzGaECHY4fHFL8NUIe9MpnB31vAmBMmiklBWNYyf9R2TBOGCMc0fdP0GUcU8BYJ4pywThT_6iFwWJ96CL-0U2m-lRy2lCm6PTsyTMUzhZW3mEPdB7PdwTvtkP5m8Zj6yBAN4BLMecEnXZ-MIOPU0a-x3D01KF6q0NR8_GJ5tH2OfoPT4nhEg
CitedBy_id crossref_primary_10_3390_ijms26072880
crossref_primary_10_1002_rmb2_12640
Cites_doi 10.1002/mabi.200600069
10.1016/j.fertnstert.2011.03.045
10.1016/j.fertnstert.2009.10.027
10.1111/j.1439-0531.2010.01739.x
10.1016/j.fertnstert.2007.10.052
10.1016/j.theriogenology.2022.10.023
10.1016/j.fertnstert.2013.03.027
10.1002/ajpa.22676
10.1038/nprot.2017.070
10.1016/j.anireprosci.2019.02.001
10.1093/humrep/den055
10.1095/biolreprod.103.023390
10.1089/ars.2017.7338
10.1016/j.anireprosci.2013.12.002
10.1002/rmb2.12411
10.1095/biolreprod.103.023093
10.1016/j.theriogenology.2016.04.036
10.1016/j.taap.2013.04.028
10.1071/RD12401
10.1088/1748-6041/11/6/065009
10.1177/1933719107303397
10.1186/s13036-018-0134-3
10.1095/biolreprod.105.042390
10.1080/10715760802695985
10.1016/j.mito.2010.09.012
10.1016/j.ydbio.2006.06.026
10.1016/S1472-6483(10)61174-1
10.1071/RD14392
10.1016/j.mce.2016.03.039
10.1007/978-1-61779-382-0
10.1210/jcem.87.1.8185
10.1242/jcs.02644
10.1002/mrd.20762
10.1055/s-2007-980222
10.1095/biolreprod53.2.295
10.1093/molehr/gat004
10.1095/biolreprod.104.028415
10.1074/jbc.M007428200
10.1007/s00441-012-1361-4
10.1262/jrd.2016-154
10.1080/03602530701468516
10.1073/pnas.0600507103
10.1177/0192623308317428
10.1002/mrd.22144
10.1017/S096719941400077X
10.1073/pnas.1218020110
10.1016/j.biomaterials.2009.06.054
10.1038/nrclinonc.2010.81
10.1093/humrep/dep228
ContentType Journal Article
Copyright COPYRIGHT 2023 MDPI AG
2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2023 by the authors. 2023
Copyright_xml – notice: COPYRIGHT 2023 MDPI AG
– notice: 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2023 by the authors. 2023
DBID AAYXX
CITATION
NPM
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQQKQ
PQUKI
7X8
7S9
L.6
5PM
DOA
DOI 10.3390/ani13060980
DatabaseName CrossRef
PubMed
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
ProQuest Central
ProQuest One
ProQuest Central Korea
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central
ProQuest One Academic Middle East (New)
ProQuest One Academic UKI Edition
ProQuest Central Essentials
ProQuest Central Korea
ProQuest One Academic Eastern Edition
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest Central (New)
ProQuest One Academic
ProQuest One Academic (New)
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitleList AGRICOLA
PubMed



MEDLINE - Academic
CrossRef
Publicly Available Content Database
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Zoology
EISSN 2076-2615
ExternalDocumentID oai_doaj_org_article_66f8b852bb174666b1d88b68e0aabe67
PMC10044016
A751912812
36978521
10_3390_ani13060980
Genre Journal Article
GeographicLocations Taiwan
Minneapolis Minnesota
New York
United States--US
GeographicLocations_xml – name: Taiwan
– name: New York
– name: Minneapolis Minnesota
– name: United States--US
GrantInformation_xml – fundername: The Ministry of Education in Taiwan
  grantid: MOE-111S0023A
– fundername: National Science and Technology Council
  grantid: MOST 109-2313-B-005-009 and MOST 110-2313-B-005-045-MY3
– fundername: Ministry of Science and Technology
  grantid: MOST 109-2313-B-005-009; MOST 110-2313-B-005-045-MY3
– fundername: Ministry of Education in Taiwan
  grantid: MOE-111S0023A
GroupedDBID 5VS
7XC
8FE
8FH
AAFWJ
AAHBH
AAYXX
ABDBF
ACUHS
AFKRA
AFPKN
ALMA_UNASSIGNED_HOLDINGS
APEBS
BENPR
CCPQU
CITATION
DIK
EAD
EAP
EPL
ESX
GROUPED_DOAJ
HYE
IAO
ITC
LK8
M48
MODMG
M~E
OK1
OZF
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
TUS
ZBA
NPM
PMFND
ABUWG
AZQEC
DWQXO
PKEHL
PQEST
PQQKQ
PQUKI
7X8
7S9
L.6
5PM
PUEGO
ID FETCH-LOGICAL-c576t-d9e0e8629ba23f7f08f686a9712b30ec41a9773657a5352777bcd72a238ee77f3
IEDL.DBID M48
ISSN 2076-2615
IngestDate Wed Aug 27 01:24:46 EDT 2025
Thu Aug 21 18:38:22 EDT 2025
Fri Jul 11 08:45:22 EDT 2025
Thu Jul 10 17:52:19 EDT 2025
Mon Jun 30 11:15:16 EDT 2025
Tue Jun 17 21:05:46 EDT 2025
Tue Jun 10 20:21:38 EDT 2025
Mon Jul 21 06:05:42 EDT 2025
Tue Jul 01 01:56:05 EDT 2025
Thu Apr 24 23:11:04 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 6
Keywords alginate encapsulation
glutathione
progesterone
reactive oxygen species
in vitro fertilization
preantral follicle
bone morphogenetic protein 15
estradiol
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c576t-d9e0e8629ba23f7f08f686a9712b30ec41a9773657a5352777bcd72a238ee77f3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-0214-6754
0000-0002-2538-4528
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/ani13060980
PMID 36978521
PQID 2791562528
PQPubID 2032438
ParticipantIDs doaj_primary_oai_doaj_org_article_66f8b852bb174666b1d88b68e0aabe67
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10044016
proquest_miscellaneous_2887622352
proquest_miscellaneous_2792502205
proquest_journals_2791562528
gale_infotracmisc_A751912812
gale_infotracacademiconefile_A751912812
pubmed_primary_36978521
crossref_citationtrail_10_3390_ani13060980
crossref_primary_10_3390_ani13060980
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20230308
PublicationDateYYYYMMDD 2023-03-08
PublicationDate_xml – month: 3
  year: 2023
  text: 20230308
  day: 8
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Animals (Basel)
PublicationTitleAlternate Animals (Basel)
PublicationYear 2023
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Hayashi (ref_18) 2017; 12
Saad (ref_32) 2019; 202
Oktem (ref_22) 2007; 14
ref_54
Lenie (ref_3) 2004; 71
Gode (ref_36) 2011; 95
Tripathi (ref_51) 2009; 43
ref_19
Borman (ref_29) 2004; 71
Hussein (ref_40) 2005; 118
Liu (ref_17) 2023; 195
Orrenius (ref_55) 2007; 39
Peng (ref_14) 2013; 110
Khazaei (ref_15) 2017; 11
Dolmans (ref_5) 2013; 99
Atwood (ref_20) 2016; 430
Joo (ref_9) 2016; 11
Hreinsson (ref_38) 2002; 87
Kreeger (ref_6) 2005; 73
Woodruff (ref_1) 2010; 7
(ref_52) 2011; 11
Filatov (ref_7) 2015; 7
Picut (ref_43) 2008; 36
Ola (ref_21) 2008; 75
Avella (ref_42) 2013; 19
Hanrahan (ref_39) 2004; 70
Agarwal (ref_50) 2005; 11
Shikanov (ref_11) 2009; 30
Jones (ref_24) 2019; 13
Rowe (ref_45) 2015; 156
Brito (ref_4) 2014; 26
Peretz (ref_28) 2013; 271
Oktay (ref_44) 1995; 53
Gasperin (ref_48) 2014; 144
Wu (ref_16) 2019; 30
Scarlet (ref_47) 2016; 28
Harada (ref_53) 2021; 20
Haidari (ref_23) 2008; 90
Rossi (ref_34) 2016; 24
Hussein (ref_37) 2006; 296
Yoshino (ref_41) 2006; 103
Skory (ref_12) 2013; 80
Green (ref_25) 2016; 86
Paulini (ref_13) 2011; 46
West (ref_8) 2007; 25
Jin (ref_26) 2010; 93
Kempisty (ref_46) 2014; 28
Salehnia (ref_31) 2016; 17
Augst (ref_10) 2006; 6
Lima (ref_35) 2012; 348
Otsuka (ref_49) 2000; 275
Meirow (ref_2) 2008; 23
Komatsu (ref_33) 2017; 63
Xu (ref_30) 2009; 24
Jalili (ref_27) 2020; 18
References_xml – volume: 6
  start-page: 623
  year: 2006
  ident: ref_10
  article-title: Alginate hydrogels as biomaterials
  publication-title: Macromol. Biosci.
  doi: 10.1002/mabi.200600069
– volume: 95
  start-page: 2274
  year: 2011
  ident: ref_36
  article-title: Influence of follicular fluid GDF9 and BMP15 on embryo quality
  publication-title: Fertil. Steril.
  doi: 10.1016/j.fertnstert.2011.03.045
– volume: 93
  start-page: 2633
  year: 2010
  ident: ref_26
  article-title: A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse
  publication-title: Fertil. Steril.
  doi: 10.1016/j.fertnstert.2009.10.027
– volume: 46
  start-page: 354
  year: 2011
  ident: ref_13
  article-title: The role of oocyte-secreted factors GDF9 and BMP15 in follicular development and oogenesis
  publication-title: Reprod. Domest. Anim.
  doi: 10.1111/j.1439-0531.2010.01739.x
– volume: 17
  start-page: 199
  year: 2016
  ident: ref_31
  article-title: Short term organ culture of mouse ovary in the medium supplemented with bone morphogenetic protein 15 and follicle stimulating hormone: A morphological, hormonal and molecular study
  publication-title: J. Reprod. Infertil.
– volume: 7
  start-page: 48
  year: 2015
  ident: ref_7
  article-title: In vitro mouse ovarian follicle growth and maturation in alginate hydrogel: Current state of the art
  publication-title: Acta Nat. (Англoязычная Версия)
– volume: 11
  start-page: 63
  year: 2017
  ident: ref_15
  article-title: Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes
  publication-title: Int. J. Fertil. Steril.
– volume: 90
  start-page: 2389
  year: 2008
  ident: ref_23
  article-title: The effect of leukemia inhibitory factor and coculture on the in vitro maturation and ultrastructure of vitrified and nonvitrified isolated mouse preantral follicles
  publication-title: Fertil. Steril.
  doi: 10.1016/j.fertnstert.2007.10.052
– volume: 195
  start-page: 159
  year: 2023
  ident: ref_17
  article-title: l-Proline improves the cytoplasmic maturation of mouse oocyte by regulating glutathione-related redox homeostasis
  publication-title: Theriogenology
  doi: 10.1016/j.theriogenology.2022.10.023
– volume: 99
  start-page: 1514
  year: 2013
  ident: ref_5
  article-title: Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue
  publication-title: Fertil. Steril.
  doi: 10.1016/j.fertnstert.2013.03.027
– volume: 156
  start-page: 665
  year: 2015
  ident: ref_45
  article-title: CYP17 genotype modifies the impact of anthropometric variation on salivary estradiol in healthy women
  publication-title: Am. J. Phys. Anthropol.
  doi: 10.1002/ajpa.22676
– volume: 12
  start-page: 1733
  year: 2017
  ident: ref_18
  article-title: Reconstitution of mouse oogenesis in a dish from pluripotent stem cells
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2017.070
– volume: 202
  start-page: 80
  year: 2019
  ident: ref_32
  article-title: Effect of plasma progesterone on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows
  publication-title: Anim. Reprod. Sci.
  doi: 10.1016/j.anireprosci.2019.02.001
– volume: 23
  start-page: 1007
  year: 2008
  ident: ref_2
  article-title: Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients
  publication-title: Hum. Reprod.
  doi: 10.1093/humrep/den055
– volume: 71
  start-page: 366
  year: 2004
  ident: ref_29
  article-title: Progesterone promotes oocyte maturation, but not ovulation, in nonhuman primate follicles without a gonadotropin surge
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.103.023390
– volume: 30
  start-page: 674
  year: 2019
  ident: ref_16
  article-title: Metabolism, nutrition, and redox signaling of hydroxyproline
  publication-title: Antioxid. Redox Signal.
  doi: 10.1089/ars.2017.7338
– volume: 144
  start-page: 72
  year: 2014
  ident: ref_48
  article-title: Expression of receptors for BMP15 is differentially regulated in dominant and subordinate follicles during follicle deviation in cattle
  publication-title: Anim. Reprod. Sci.
  doi: 10.1016/j.anireprosci.2013.12.002
– volume: 20
  start-page: 505
  year: 2021
  ident: ref_53
  article-title: Improved developmental potential of mouse vitrified-warmed oocytes achieved by culturing in recovery medium with glutathione ethyl ester (GSH-OEt)
  publication-title: Reprod. Med. Biol.
  doi: 10.1002/rmb2.12411
– volume: 70
  start-page: 900
  year: 2004
  ident: ref_39
  article-title: Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries)
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.103.023093
– volume: 86
  start-page: 229
  year: 2016
  ident: ref_25
  article-title: In vitro culture methods of preantral follicles
  publication-title: Theriogenology
  doi: 10.1016/j.theriogenology.2016.04.036
– volume: 271
  start-page: 249
  year: 2013
  ident: ref_28
  article-title: Bisphenol A down-regulates rate-limiting Cyp11a1 to acutely inhibit steroidogenesis in cultured mouse antral follicles
  publication-title: Toxicol. Appl. Pharmacol.
  doi: 10.1016/j.taap.2013.04.028
– volume: 26
  start-page: 915
  year: 2014
  ident: ref_4
  article-title: Three-dimensional systems for in vitro follicular culture: Overview of alginate-based matrices
  publication-title: Reprod. Fertil. Dev.
  doi: 10.1071/RD12401
– volume: 11
  start-page: 065009
  year: 2016
  ident: ref_9
  article-title: The effect of collagen hydrogel on 3D culture of ovarian follicles
  publication-title: Biomed. Mater.
  doi: 10.1088/1748-6041/11/6/065009
– volume: 14
  start-page: 358
  year: 2007
  ident: ref_22
  article-title: The role of extracellular matrix and activin-A in in vitro growth and survival of murine preantral follicles
  publication-title: Reprod. Sci.
  doi: 10.1177/1933719107303397
– volume: 13
  start-page: 2
  year: 2019
  ident: ref_24
  article-title: Follicle development as an orchestrated signaling network in a 3D organoid
  publication-title: J. Biol. Eng.
  doi: 10.1186/s13036-018-0134-3
– volume: 73
  start-page: 942
  year: 2005
  ident: ref_6
  article-title: Regulation of mouse follicle development by follicle-stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.105.042390
– volume: 43
  start-page: 287
  year: 2009
  ident: ref_51
  article-title: Intracellular levels of hydrogen peroxide and nitric oxide in oocytes at various stages of meiotic cell cycle and apoptosis
  publication-title: Free Radic. Res.
  doi: 10.1080/10715760802695985
– volume: 11
  start-page: 797
  year: 2011
  ident: ref_52
  article-title: Mitochondrial function in the human oocyte and embryo and their role in developmental competence
  publication-title: Mitochondrion
  doi: 10.1016/j.mito.2010.09.012
– volume: 296
  start-page: 514
  year: 2006
  ident: ref_37
  article-title: Oocyte-secreted factors enhance oocyte developmental competence
  publication-title: Dev. Biol.
  doi: 10.1016/j.ydbio.2006.06.026
– volume: 11
  start-page: 641
  year: 2005
  ident: ref_50
  article-title: Oxidative stress and its implications in female infertility—A clinician’s perspective
  publication-title: Reprod. Biomed. Online
  doi: 10.1016/S1472-6483(10)61174-1
– volume: 28
  start-page: 1340
  year: 2016
  ident: ref_47
  article-title: Expression and immunolocalisation of follicle-stimulating hormone receptors in gonads of newborn and adult female horses
  publication-title: Reprod. Fertil. Dev.
  doi: 10.1071/RD14392
– volume: 430
  start-page: 33
  year: 2016
  ident: ref_20
  article-title: The spatiotemporal hormonal orchestration of human folliculogenesis, early embryogenesis and blastocyst implantation
  publication-title: Mol. Cell. Endocrinol.
  doi: 10.1016/j.mce.2016.03.039
– ident: ref_54
  doi: 10.1007/978-1-61779-382-0
– volume: 87
  start-page: 316
  year: 2002
  ident: ref_38
  article-title: Growth differentiation factor-9 promotes the growth, development, and survival of human ovarian follicles in organ culture
  publication-title: J. Clin. Endocrinol. Metab.
  doi: 10.1210/jcem.87.1.8185
– volume: 118
  start-page: 5257
  year: 2005
  ident: ref_40
  article-title: Oocytes prevent cumulus cell apoptosis by maintaining a morphogenic paracrine gradient of bone morphogenetic proteins
  publication-title: J. Cell Sci.
  doi: 10.1242/jcs.02644
– volume: 75
  start-page: 89
  year: 2008
  ident: ref_21
  article-title: Effects of gonadotrophins, growth hormone, and activin A on enzymatically isolated follicle growth, oocyte chromatin organization, and steroid secretion
  publication-title: Mol. Reprod. Dev. Inc. Gamete Res.
  doi: 10.1002/mrd.20762
– volume: 25
  start-page: 287
  year: 2007
  ident: ref_8
  article-title: Engineering the follicle microenvironment
  publication-title: Semin. Reprod. Med.
  doi: 10.1055/s-2007-980222
– volume: 53
  start-page: 295
  year: 1995
  ident: ref_44
  article-title: Proliferating cell nuclear antigen marks the initiation of follicular growth in the rat
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod53.2.295
– volume: 18
  start-page: 517
  year: 2020
  ident: ref_27
  article-title: Effects of sodium alginate capsules as 3D scaffolds on hormones and genes expression in preantral follicles of mice compared to 2D medium: An experimental study
  publication-title: Int. J. Reprod. BioMedicine
– volume: 19
  start-page: 279
  year: 2013
  ident: ref_42
  article-title: The molecular basis of gamete recognition in mice and humans
  publication-title: MHR Basic Sci. Reprod. Med.
  doi: 10.1093/molehr/gat004
– volume: 71
  start-page: 1730
  year: 2004
  ident: ref_3
  article-title: A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.104.028415
– volume: 275
  start-page: 39523
  year: 2000
  ident: ref_49
  article-title: Bone morphogenetic protein-15: Identification of target cells and biological functions
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M007428200
– volume: 348
  start-page: 225
  year: 2012
  ident: ref_35
  article-title: BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development
  publication-title: Cell Tissue Res.
  doi: 10.1007/s00441-012-1361-4
– volume: 63
  start-page: 271
  year: 2017
  ident: ref_33
  article-title: The concentration-dependent effect of progesterone on follicle growth in the mouse ovary
  publication-title: J. Reprod. Dev.
  doi: 10.1262/jrd.2016-154
– volume: 39
  start-page: 443
  year: 2007
  ident: ref_55
  article-title: Reactive oxygen species in mitochondria-mediated cell death
  publication-title: Drug Metab. Rev.
  doi: 10.1080/03602530701468516
– volume: 103
  start-page: 10678
  year: 2006
  ident: ref_41
  article-title: A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0600507103
– volume: 28
  start-page: 419
  year: 2014
  ident: ref_46
  article-title: Association between the expression of LHR, FSHR and CYP19 genes, cellular distribution of encoded proteins and proliferation of porcine granulosa cells in real-time
  publication-title: J. Biol. Regul. Homeost. Agents
– volume: 36
  start-page: 674
  year: 2008
  ident: ref_43
  article-title: Ovarian follicle counts using proliferating cell nuclear antigen (PCNA) and semi-automated image analysis in rats
  publication-title: Toxicol. Pathol.
  doi: 10.1177/0192623308317428
– volume: 80
  start-page: 132
  year: 2013
  ident: ref_12
  article-title: Microarray analysis identifies COMP as the most differentially regulated transcript throughout in vitro follicle growth
  publication-title: Mol. Reprod. Dev.
  doi: 10.1002/mrd.22144
– volume: 24
  start-page: 1
  year: 2016
  ident: ref_34
  article-title: The bone morphogenetic protein system and the regulation of ovarian follicle development in mammals
  publication-title: Zygote
  doi: 10.1017/S096719941400077X
– volume: 110
  start-page: E776
  year: 2013
  ident: ref_14
  article-title: Growth differentiation factor 9: Bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1218020110
– volume: 30
  start-page: 5476
  year: 2009
  ident: ref_11
  article-title: Interpenetrating fibrin–alginate matrices for in vitro ovarian follicle development
  publication-title: Biomaterials
  doi: 10.1016/j.biomaterials.2009.06.054
– ident: ref_19
– volume: 7
  start-page: 466
  year: 2010
  ident: ref_1
  article-title: The Oncofertility Consortium—Addressing fertility in young people with cancer
  publication-title: Nat. Rev. Clin. Oncol.
  doi: 10.1038/nrclinonc.2010.81
– volume: 24
  start-page: 2531
  year: 2009
  ident: ref_30
  article-title: In vitro grown human ovarian follicles from cancer patients support oocyte growth
  publication-title: Hum. Reprod.
  doi: 10.1093/humrep/dep228
SSID ssj0000753151
Score 2.2697682
Snippet Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein...
Multilayered secondary follicles were encapsulated and cultured for 12 days in a 3D culture system by using alginate supplemented with bone morphogenetic...
Simple SummaryMultilayered secondary follicles were encapsulated and cultured for 12 days in a 3D culture system by using alginate supplemented with bone...
SourceID doaj
pubmedcentral
proquest
gale
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 980
SubjectTerms alginate encapsulation
alginates
bone morphogenetic protein 15
Bone morphogenetic proteins
Cancer therapies
estradiol
Fertility
follicular development
Gene expression
glutathione
Growth factors
Hydrogels
in vitro fertilization
Infertility
metaphase
mice
oocytes
ovarian follicles
Ovaries
Oxidative stress
preantral follicle
reactive oxygen species
secretion
survival rate
Transforming growth factors
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1bi9UwEA6yIPgi3q2uEmHBC5Rtk-bSxz3iYRWO7oMriy-hSadsZWlle87_dybtHloUffGtNJOQTiaZb5rMF8aOwGgjtNRpaBQGKGUNuA5KlQqRSwBfVT5QcvLmsz49Lz5dqIvZVV90JmykBx4Vd6x1Y71VwnvEzoi1fV5b67WFDNsBHfPI0efNgqkf4_acRF82JuRJjOuPq67F5VpnJRFAzlxQZOr_fT2eOaTlYcmZ91nfY3cn2MhPxu7eZ7ege8Buf-_jT_GH7Muq74BvelRajxZBiYn8jBgY2o7nir9Zbc7SXL3l4y8EGPjHjn9rt9c91tkNwNdEzR12V7Hy0A6P2Pn6w9f3p-l0VUIaMGDYpnUJGWBwUvpKyMY0mW201VVpcuFlBqHI8dlIrUxFfC7GGB9qI1DYAhjTyMfsoMOePmVciQwxSd1UFhDNNVVpiyLIIoDy2FhuEvbuRnsuTDzidJ3FlcN4glTtZqpO2NFe-OdIn_FnsRUNw16EOK_jC7QEN1mC-5clJOw1DaKjmYkdCtWUYICfRRxX7sQgWqWNQ5Gww4UkzqiwLL4xAzfN6MEJU-YULAqbsFf7YqpJp9Q6wNEiGUSUlLr8FxlL_kfgICTsyWhZ-8-WGmN6xFMJswubW-hlWdK1l5ETPBL_IXx_9j80-ZzdEYjl4lE7e8gOttc7eIHYa-tfxmn2C57xKcA
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9swEBdby2AvY99z1w0NCvsAU1uyJflpNKOhG6QLYx1lL8aSz5uh2G2c_P-7sxUvZqNvwTqFs3Qfv5Olnxg7Aq20UFKFrkqxQMlKwDgo01CIWALYorCODicvztXZRfLlMr30C26d31a5jYl9oC5bR2vkx0JnMYF1YT5e34R0axR9XfVXaNxl-xiCDRZf-7PT8-W3cZUFE6LEnDYczJNY3x8XTY1hW0UZEUHupKKesf_fuLyTmKabJney0Pwhe-DhIz8Z5vsRuwPNY3bvZ9svjj9hX2dtA3zR4uC1aBl0QJEviYmhbnic8nezxTKM0_d8WEqAjn9u-I96vWqxz6YDPieKbre56jt3dfeUXcxPv386C_2VCaHDwmEdlhlEgEVKZgshK11FplJGFZmOhZURuCTG31qqVBfE66K1tq7UAoUNgNaVfMb2GtT0BeOpiBCblFVhAFFdVWQmSZxMHKQW_yzWAfuwHb3ceT5xutbiKse6goY63xnqgB2NwtcDjcb_xWY0DaMIcV_3D9rVr9y7Uq5UZaxJhUUlEqy-bFwaY5WBCC0LFCr2liYxJw9FhVzhDxrgaxHXVX6iEbXSB0QRsMOJJHqWmzZvzSD3nt3lf-0wYG_GZupJu9UawNkiGUSWdIT5FhlDeUjgJATs-WBZ42tLhbU94qqAmYnNTcZl2tLUv3tu8J4AEGH8we26v2T3BaK1fjOdOWR769UGXiG6WtvX3oX-ABsNIn0
  priority: 102
  providerName: ProQuest
Title Bone Morphogenetic Protein 15 (BMP-15) Improves In Vitro Mouse Folliculogenesis
URI https://www.ncbi.nlm.nih.gov/pubmed/36978521
https://www.proquest.com/docview/2791562528
https://www.proquest.com/docview/2792502205
https://www.proquest.com/docview/2887622352
https://pubmed.ncbi.nlm.nih.gov/PMC10044016
https://doaj.org/article/66f8b852bb174666b1d88b68e0aabe67
Volume 13
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3di9QwEB-OOwRfxG-r51LhQBCqbdJ89EHkVm45hT0OceXwpTRpqpWl1e0u6H_vTNpdtnrcW2kmJZnMZOaXZmYATpySikkuI1sJBChZ6XAf5CJiLOHOmaIwloKT5xfyfJF-vBJXB7AtxjkwsLsW2lE9qcVq-fr3rz_vUOHfEuJEyP6maGrciWWcacTuR2iSFGnofPDzf_R_63jiSzEyxO0RogbRx-r9239knXwS__-36j1bNb5HuWeYZnfhzuBRhqe9CNyDA9fch1tfW39e_gBm07Zx4bxFfrYoLBSzGF5Scoa6CRMR9ocKrgs_NOGXer1qkXTTuXBGybrtZun7dHX3EBazs8_vz6OheEJkEUKsozJzsUO4kpmC8UpVsa6klkWmEmZ47Gya4LPiUqiCMrwopYwtFUNi7ZxSFX8Ehw0O8AmEgsXopZRVoR36d1WR6TS1PLVOGPxYogJ4tWVabofM4lTgYpkjwiAO53scDuBkR_yzT6hxPdmUuL8joSzY_kW7-pYPSpVLWWmjBTM4iBRxmElKrY3ULkYZcxIH9pLWLifpwQHZYgg5wGlR1qv8VKH_Sr8SWQDHI0rUMTtu3q5-vhXRnKksIfjIdAAvds3Uk-6tNQ5Xi2jQx6Rg5htoNFkkhosQwONeoHbT5hJRPnpYAeiRqI34Mm5p6u8-S7hPBYgO_dObp_YMbjP02_y1On0Mh-vVxj1HP2ttJnA0Pbu4_DTx5xQTr1F_AeEwJW0
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR1db9Mw8DQ6IXhBfBMYEKQhPqRoiZ3YzgNCK6xq2VoqtKGJlxA7DlSaktG0QvwpfiN3SVoagfa2tyg-R-e78304vjuAXSuFZIILz-QRBihxZlEP8shjLODW6jTVhpKTxxMxPAk_nEanW_B7lQtD1ypXOrFW1Flp6Ix8j8k4IGedqbfnPzzqGkV_V1ctNBqxOLS_fmLIVr0ZvUf-PmdscHD8bui1XQU8g771wsti61v042OdMp7L3Fe5UCKNZcA0960JA3yWXEQypdInUkptMskQWFkrZc7xu1dgO-QYyvRgu38wmX5an-qgAeZoQ5tEQM5jfy8tZmgmhB9T4ckN01d3CPjXDmwYwu4lzQ2rN7gJN1p31d1v5OsWbNniNlz9UtaH8XfgY78srDsukVklSiIlRLpTqvwwK9wgcl_2x1MviF65zdGFrdxR4X6eLeYlzllW1h1QSXCzPKsnV7PqLpxcCjHvQa9ATB-AGzEffaEsT5VFLzJPYxWGhofGRho_FkgHXq-ol5i2fjm10ThLMI4hUicbpHZgdw183pTt-D9Yn9iwBqFa2_WLcv4tabduIkSutIqYRiRCjPZ0kCmlhbI-SrIViNgLYmJCGgERMmmb2IDLotpayb5EL5l-WDIHdjqQuJNNd3glBkmrSarkr9w78Gw9TDPpdlxhkVsEg54spUxfAKPI7jFkggP3G8laL5uLWOICAwdUR-Y6dOmOFLPvdS3yuuAghg0PL8b9KVwbHo-PkqPR5PARXGfoKdYX-dQO9BbzpX2Mnt1CP2m3kwtfL3sH_wF_eV5I
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR1db9Mw8DQ6gXhBfBMYYKQhPqSoiZ3YzgNCK1u1MloqxKaJl5A4DlSaktG0Qvw1fh13SVoagfa2tyg-R_Z9-O6c-wDYtUoqLoV0TR6igxJlFs9BEbqc-8LaNElSQ8nJ44k8PA7en4anW_B7lQtDYZWrM7E-qLPS0B15n6vIJ2Od637ehkVM94dvz3-41EGK_rSu2mk0LHJkf_1E9616M9pHWj_nfHjw-d2h23YYcA3a2Qs3i6xn0aaP0oSLXOWezqWWSaR8ngrPmsDHZyVkqBIqg6KUSk2mOAJra5XKBX73Cmwr9Iq8HmwPDibTT-sbHlTGAvVpkxQoROT1k2KGKkN6ERWh3FCDdbeAf3XChlLsBmxuaMDhTbjRmq5sr-G1W7Bli9tw9UtZX8zfgY-DsrBsXCLhSuRKSo5kU6oCMSuYH7KXg_HU9cNXrLnGsBUbFexktpiXOGdZWTak8uBmeVZPrmbVXTi-FGTeg16BK30ALOQe2kVZnmiLFmWeRDoIjAiMDVP8mK8ceL3CXmzaWubUUuMsRp-GUB1voNqB3TXweVPC4_9gAyLDGoTqbtcvyvm3uBXjWMpcpzrkKS4iQM8v9TOtU6mth1xtJS7sBRExptMBF2SSNskBt0V1tuI9hRYz_bzkDux0IFGqTXd4xQZxe6pU8V8ZcODZephmUqRcYZFaBINWLaVPXwCjSQdyJIID9xvOWm9byEjhBn0HdIfnOnjpjhSz73Vd8rr4ILoQDy9e-1O4hpIbfxhNjh7BdY5GYx3Tp3egt5gv7WM08hbpk1aaGHy9bAH-A_tKYn0
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Bone+Morphogenetic+Protein+15+Improves+In+Vitro+Mouse+Folliculogenesis&rft.jtitle=Animals+%28Basel%29&rft.au=Jitjumnong%2C+Jakree&rft.au=Tang%2C+Pin-Chi&rft.date=2023-03-08&rft.pub=MDPI+AG&rft.issn=2076-2615&rft.eissn=2076-2615&rft.volume=13&rft.issue=6&rft_id=info:doi/10.3390%2Fani13060980&rft.externalDocID=A751912812
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2076-2615&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2076-2615&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2076-2615&client=summon