Bone Morphogenetic Protein 15 (BMP-15) Improves In Vitro Mouse Folliculogenesis

Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle sur...

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Published inAnimals (Basel) Vol. 13; no. 6; p. 980
Main Authors Jitjumnong, Jakree, Tang, Pin-Chi
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 08.03.2023
MDPI
Subjects
alginate encapsulation
alginates
bone morphogenetic protein 15
Bone morphogenetic proteins
Cancer therapies
estradiol
Fertility
follicular development
Gene expression
glutathione
Growth factors
Hydrogels
in vitro fertilization
Infertility
metaphase
mice
oocytes
ovarian follicles
Ovaries
Oxidative stress
preantral follicle
reactive oxygen species
secretion
survival rate
Transforming growth factors
Taiwan
Minneapolis Minnesota
New York
United States > US
alginate encapsulation
glutathione
progesterone
reactive oxygen species
in vitro fertilization
preantral follicle
bone morphogenetic protein 15
estradiol
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Summary:Multilayered secondary follicles were encapsulated in a 0.5% alginate matrix and cultured in a 3D culture system supplemented with bone morphogenetic protein 15 (BMP-15; 15 ng/mL) for 12 days. The in vitro development of ovarian follicles was evaluated. On day 12, the follicle diameter, follicle survival rate, and antrum formation rate were significantly higher for follicles cultured in BMP-15-supplemented medium than those cultured in regular medium. The percentage of ovulated metaphase II oocytes retrieved from follicles cultured in BMP-15-supplemented medium was greater than that of oocytes retrieved from follicles cultured in regular medium. The secretion of P4 was significantly higher on days 6, 8, and 10 in follicles cultured in BMP-15-supplemented medium. The result for E2 tended toward significance on day 12. Intracellular reactive oxygen species levels were higher and glutathione levels were lower in mature oocytes from the in vitro culture than in mature oocytes from an in vivo control. A 3D culture system using an alginate matrix and supplemented with BMP-15 effectively improves the outcomes of in vitro ovarian follicle culture.
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ISSN:2076-2615
2076-2615
DOI:10.3390/ani13060980