Mask family proteins ANKHD1 and ANKRD17 regulate YAP nuclear import and stability

Mask family proteins were discovered in to promote the activity of the transcriptional coactivator Yorkie (Yki), the sole fly homolog of mammalian YAP (YAP1) and TAZ (WWTR1). The molecular function of Mask, or its mammalian homologs Mask1 (ANKHD1) and Mask2 (ANKRD17), remains unclear. Mask family pr...

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Published ineLife Vol. 8
Main Authors Sidor, Clara, Borreguero-Munoz, Nerea, Fletcher, Georgina C, Elbediwy, Ahmed, Guillermin, Oriane, Thompson, Barry J
Format Journal Article
LanguageEnglish
Published England eLife Science Publications, Ltd 29.10.2019
eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
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Summary:Mask family proteins were discovered in to promote the activity of the transcriptional coactivator Yorkie (Yki), the sole fly homolog of mammalian YAP (YAP1) and TAZ (WWTR1). The molecular function of Mask, or its mammalian homologs Mask1 (ANKHD1) and Mask2 (ANKRD17), remains unclear. Mask family proteins contain two ankyrin repeat domains that bind Yki/YAP as well as a conserved nuclear localisation sequence (NLS) and nuclear export sequence (NES), suggesting a role in nucleo-cytoplasmic transport. Here we show that Mask acts to promote nuclear import of Yki, and that addition of an ectopic NLS to Yki is sufficient to bypass the requirement for Mask in Yki-driven tissue growth. Mammalian Mask1/2 proteins also promote nuclear import of YAP, as well as stabilising YAP and driving formation of liquid droplets. Mask1/2 and YAP normally colocalise in a granular fashion in both nucleus and cytoplasm, and are co-regulated during mechanotransduction.
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content type line 23
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.48601