Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials

• Published in: Journal of applied oral science Vol. 26; p. e20170562
• Main Authors: Peralta, Sonia Luque, Leles, Sávio Bisinoto de, Dutra, André Lindemann, Guimarães, Victoria Burmann da Silva, Piva, Evandro, Lund, Rafael Guerra
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade De Odontologia De Bauru - USP 01.01.2018; University of São Paulo
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bisphenol A-Glycidyl Methacrylate - chemistry; Bisphenol A-Glycidyl Methacrylate - pharmacology; Cattle; Cell Survival - drug effects; Composite Resins - chemistry; Composite Resins - pharmacology; Dental Leakage; Dental Restoration, Temporary - methods; DENTISTRY, ORAL SURGERY & MEDICINE; Enterococcus faecalis - drug effects; Fibroblasts - drug effects; Glass Ionomer Cements - chemistry; Glass Ionomer Cements - pharmacology; Hardness Tests; Materials Testing; Methylmethacrylates - chemistry; Methylmethacrylates - pharmacology; Mice; Microbiology; Original; Polymethacrylic Acids - chemistry; Polymethacrylic Acids - pharmacology; Reproducibility of Results; Root Canal Filling Materials - chemistry; Root Canal Filling Materials - pharmacology; Solubility; Streptococcus mutans - drug effects; Temporary dental restoration; Tensile Strength; Time Factors; Zinc Oxide-Eugenol Cement - chemistry; Zinc Oxide-Eugenol Cement - pharmacology; Dental leakage; Microbiology; Temporary dental restoration; Solubility
• ISSN: 1678-7757; 1678-7765; 1678-7765
• DOI: 10.1590/1678-7757-2017-0562

The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.