Analysis of the bone ultrastructure around biodegradable Mg–xGd implants using small angle X-ray scattering and X-ray diffraction

• Published in: Acta biomaterialia Vol. 101; no. 1; pp. 637 - 645
• Main Authors: Zeller-Plumhoff, Berit, Malich, Carina, Krüger, Diana, Campbell, Graeme, Wiese, Björn, Galli, Silvia, Wennerberg, Ann, Willumeit-Römer, Regine, Wieland, D.C. Florian
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2020; Elsevier BV
• Subjects: Absorbable Implants; alloys; Animals; Apatite; Biodegradability; Biodegradable magnesium implants; Biodegradation; Biomaterials Science; Biomaterialvetenskap; Blood Platelets - drug effects; Bone and Bones - ultrastructure; Bone implants; Bone matrix; Bone ultrastructure; Bone-implant; Bone-implant interface; Crystallites; Crystallization; Crystals; Degradation; Dentistry; Durapatite - pharmacology; Engineering; Gadolinium; Gadolinium - pharmacology; Hydroxyapatite; in-vitro; interface; Lattice theory; Magnesium; Magnesium - pharmacology; Magnesium base alloys; Male; Materials Science; Mechanical properties; Metals; Mineralization; nanostructure; Odontologi; Protective coatings; Rats, Sprague-Dawley; Small angle X ray scattering; Statistical analysis; Surgical implants; Titanium; Titanium - pharmacology; Transplants & implants; Ultrastructure; X-Ray Diffraction; X-ray scattering; Biodegradable magnesium implants; Bone ultrastructure; HAP; Mg; XRD; Bone-implant interface; SAXS
• ISSN: 1742-7061; 1878-7568; 1878-7568
• DOI: 10.1016/j.actbio.2019.11.030

Magnesium alloys are increasingly researched as temporary biodegradable metal implants in bone applications due to their mechanical properties which are more similar to bone than conventional implant metals and the fact that Magnesium occurs naturally within the body. However, the degradation processes in vivo and in particular the interaction of the bone with the degrading material need to be further investigated. In this study we are presenting the first quantitative comparison of the bone ultrastructure formed at the interface of biodegradable Mg–5Gd and Mg–10Gd implants and titanium and PEEK implants after 4, 8 and 12 weeks healing time using two-dimensional small angle X-ray scattering and X-ray diffraction. Differences in mineralization, orientation and thickness of the hydroxyapatite are assessed. We find statistically significant (p < 0.05) differences for the lattice spacing of the (310)-reflex of hydroxyapatite between titanium and Mg–xGd materials, as well as for the (310) crystal size between titanium and Mg–5Gd, indicating a possible deposition of Mg within the bone matrix. The (310) lattice spacing and crystallite size further differ significantly between implant degradation layer and surrounding bone (p < 0.001 for Mg–10Gd), suggesting apatite formation with significant amounts of Gd and Mg within the degradation layer. Biodegradable Magnesium-based alloys are emerging as a viable alternative for temporary bone implant applications. However, in order to understand if the degradation of the implant material influences the bone ultrastructure, it is necessary to study the bone structure using high-resolution techniques. We have therefore employed 2D small angle X-ray scattering and X-ray diffraction to study the bone ultrastructure surrounding Magnesium–Gadolinium alloys as well as Titanium and PEEK alloys at three different healing times. This is the first time, that the bone ultrastructure around these materials is directly compared and that a statistical evaluation is performed. We found differences indicating a possible deposition of Mg within the bone matrix as well as a local deposition of Mg and/or Gd at the implant site. The raw/processed data required to reproduce these findings cannot be shared at this time as the data also forms part of an ongoing study. [Display omitted]