3,3'-diethylthiatricarbocyanine iodide: a highly sensitive chiroptical reporter of DNA helicity and sequence

• Published in: International journal of molecular sciences Vol. 12; no. 11; pp. 8052 - 8062
• Main Authors: Choi, Jung Kyu, D'Urso, Alessandro, Trauernicht, Mitch, Shabbir-Hussain, Murtaza, Holmes, Andrea E, Balaz, Milan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.11.2011; Molecular Diversity Preservation International (MDPI)
• Subjects: Benzothiazoles - chemistry; Carbocyanines - chemistry; Circular Dichroism; Cy7 cyanine dye; DNA recognition; DNA, B-Form - chemistry; DNA, Z-Form - chemistry; Dyes; left-handed Z-DNA; Polydeoxyribonucleotides - chemistry; Protein Binding; sensing; Sequence Analysis, DNA; Structure-Activity Relationship; United States > US; sensing; left-handed Z-DNA; DNA recognition; Cy7 cyanine dye; circular dichroism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms12118052

Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.