The effect of temozolomide/poly(lactide-co-glycolide) (PLGA)/nano-hydroxyapatite microspheres on glioma U87 cells behavior

• Published in: International journal of molecular sciences Vol. 13; no. 1; pp. 1109 - 1125
• Main Authors: Zhang, Dongyong, Tian, Ang, Xue, Xiangxin, Wang, Mei, Qiu, Bo, Wu, Anhua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2012; Molecular Diversity Preservation International (MDPI)
• Subjects: Antineoplastic Agents, Alkylating - chemistry; Antineoplastic Agents, Alkylating - toxicity; Apoptosis; Apoptosis - drug effects; Biocompatibility; Brain cancer; Brain tumors; Cell Line, Tumor; Cell proliferation; controlled release; Dacarbazine - analogs & derivatives; Dacarbazine - chemistry; Dacarbazine - toxicity; Data processing; Differential scanning calorimetry; Drug Carriers - chemistry; Drug delivery; Drugs; Durapatite - chemistry; Flow cytometry; Glioma; Glioma - metabolism; Glioma - pathology; Glioma cells; Humans; Hydroxyapatite; Integrin alphaVbeta3 - metabolism; Integrins; Investigations; Lactic Acid - chemistry; Microspheres; Morphology; Nanostructures - chemistry; Oil; Poly (lactide-co-glycolide) PLGA; Polyglycolic Acid - chemistry; polylactide-co-glycolide; Polymerase chain reaction; Scanning electron microscopy; temozolomide; temozolomide (TMZ); Western blotting; X-ray diffraction; China; hydroxyapatite; glioma; controlled release; temozolomide (TMZ); Poly (lactide-co-glycolide) PLGA
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms13011109

In this study, we investigated the effects of temozolomide (TMZ)/Poly (lactide-co-glycolide)(PLGA)/nano-hydroxyapatite microspheres on the behavior of U87 glioma cells. The microspheres were fabricated by the "Solid/Water/Oil" method, and they were characterized by using X-Ray diffraction, scanning electron microscopy and differential scanning calorimetry. The proliferation, apoptosis and invasion of glioma cells were evaluated by MTT, flow cytometry assay and Transwell assay. The presence of the key invasive gene, α(V)β3 integrin, was detected by the RT-PCR and Western blot method. It was found that the temozolomide/PLGA/nano-hydroxyapatite microspheres have a significantly diminished initial burst of drug release, compared to the TMZ laden PLGA microspheres. Our results suggest they can significantly inhibit the proliferation and invasion of glioma cells, and induce their apoptosis. Additionally, α(V)β3 integrin was also reduced by the microspheres. These data suggest that by inhibiting the biological behavior of glioma cells in vitro, the newly designed temozolomide/PLGA/nano-hydroxyapatite microspheres, as controlled drug release carriers, have promising potential in treating glioma.