Loss of Marek's disease virus tumorigenicity is associated with truncation of RNAs transcribed within BamHI-H

The attenuation of Marek's disease virus (MDV) is associated with loss of pathogenicity and tumorigenicity. Previous studies have demonstrated a strong correlation between attentuation and amplification of a specific sequence located within the MDV terminal and internal repeats. We recently rep...

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Bibliographic Details
Published inJournal of Virology Vol. 63; no. 10; pp. 4129 - 4135
Main Authors Bradley, G. (University of South Florida School of Medicine, Tampa, FL), Lancz, G, Tanaka, A, Nonyama, M
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.1989
Subjects
Animals
ARN
Base Sequence
Biological and medical sciences
Chick Embryo
DNA, Viral - analysis
ENFERMEDAD DE MAREK
Fundamental and applied biological sciences. Psychology
Gene Amplification
Genes, Viral
Genetics
Herpesvirus 2, Gallid - genetics
Herpesvirus 2, Gallid - pathogenicity
HERPETOVIRIDAE
MALADIE DE MAREK
Microbiology
NUCLEOTIDE
NUCLEOTIDOS
Peptide Fragments - analysis
PODER PATOGENO
POUVOIR PATHOGENE
Proto-Oncogenes
RNA, Viral - analysis
Transcription, Genetic
Virology
Gammaherpesvirinae
Marek disease virus
RNA
Transcription
Pathogenesis
Herpesviridae
Tumorigenicity
Host virus relation
Truncation
Virus
Northern blotting
Attenuation
Isolation
Southern blotting
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Summary:The attenuation of Marek's disease virus (MDV) is associated with loss of pathogenicity and tumorigenicity. Previous studies have demonstrated a strong correlation between attentuation and amplification of a specific sequence located within the MDV terminal and internal repeats. We recently reported that the regions containing the amplified sequences, the BamHI D and H fragments, were transcriptionally active. However, differential transcription activity was observed to exist between attenuated and pathogenic MDV strains. Specifically, a major transcript of 1.8 kilobases was found to be produced by pathogenic MDV and not by attenuated MDV. We now report that the disappearance of this transcript is concomitant with the production of a 0.4-kilobase RNA, an RNA resulting from the truncation of the tumorigenicity-related transcript
Bibliography:L73
9011938
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ISSN:0022-538X
1098-5514
DOI:10.1128/jvi.63.10.4129-4135.1989