Selective Translation of Leaderless mRNAs by Specialized Ribosomes Generated by MazF in Escherichia coli

• Published in: Cell Vol. 147; no. 1; pp. 147 - 157
• Main Authors: Vesper, Oliver, Amitai, Shahar, Belitsky, Maria, Byrgazov, Konstantin, Kaberdina, Anna Chao, Engelberg-Kulka, Hanna, Moll, Isabella
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.09.2011
• Subjects: Adaptations; Base Sequence; Codon, Initiator; Codons; DNA-Binding Proteins - metabolism; Endoribonucleases - metabolism; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Gene Expression Regulation, Bacterial; Lead; messenger RNA; Models, Molecular; Molecular Sequence Data; Nucleotides; Protein Biosynthesis; protein subunits; ribosomal proteins; Ribosomal Proteins - genetics; ribosomal RNA; Ribosomal subunits; Ribosome Subunits, Small, Bacterial - metabolism; Ribosomes; RNA, Bacterial - chemistry; RNA, Bacterial - metabolism; RNA, Messenger - metabolism; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - metabolism; rRNA 16S; start codon; Stress, Physiological; Toxins; translation (genetics); Translation initiation
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2011.07.047

Escherichia coli ( E. coli) mazEF is a stress-induced toxin-antitoxin (TA) module. The toxin MazF is an endoribonuclease that cleaves single-stranded mRNAs at ACA sequences. Here, we show that MazF cleaves at ACA sites at or closely upstream of the AUG start codon of some specific mRNAs and thereby generates leaderless mRNAs. Moreover, we provide evidence that MazF also targets 16S rRNA within 30S ribosomal subunits at the decoding center, thereby removing 43 nucleotides from the 3′ terminus. As this region comprises the anti-Shine-Dalgarno (aSD) sequence that is required for translation initiation on canonical mRNAs, a subpopulation of ribosomes is formed that selectively translates the described leaderless mRNAs both in vivo and in vitro. Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli. [Display omitted] ► MazF activity causes ribosome heterogeneity in response to stress ► MazF generates a subpopulation of ribosomes selective for leaderless mRNAs ► MazF cuts upstream of AUG start codons, generating specific leaderless mRNAs ► Thus, MazF triggers a posttranscriptional stress response pathway in bacteria A stress-induced endoribonuclease cleaves 16S rRNA in a site-specific manner, leading to ribosomes that can translate only specific mRNAs that have been cleaved at their 5′ end by the same RNase.