A semi‐dominant mutation in the ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana

• Published in: The Plant journal : for cell and molecular biology Vol. 56; no. 6; pp. 881 - 890
• Main Authors: Imai, Akihiro, Komura, Mio, Kawano, Eri, Kuwashiro, Yoshitaka, Takahashi, Taku
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2008; Blackwell
• Subjects: ACL5; Alleles; Amino Acid Sequence; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; Botany; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; DNA, Bacterial - genetics; DNA, Plant - genetics; dwarf; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes, Plant; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genotype & phenotype; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Phenotype; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; polyamine; Proteins; Pteridophyta, spermatophyta; ribosomal protein; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Sequence Alignment; translation; Vegetals; Polyamine; Arabidopsis; Ribosomal protein; Arabidopsis thaliana; Dwarfism; Phenotype; Gene; Cruciferae; ACL5; Dicotyledones; Angiospermae; translation; Spermatophyta; dwarf; Mutation
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/j.1365-313X.2008.03647.x

Summary Disruption of the Arabidopsis thaliana ACAULIS5 (ACL5) gene, which has recently been shown to encode thermospermine synthase, results in a severe dwarf phenotype. A previous study showed that sac51‐d, a dominant suppressor mutant of acl5‐1, has a premature termination codon in an upstream open reading frame (ORF) of SAC51, which encodes a putative transcription factor, and suggested the involvement of upstream ORF‐mediated translational control in ACL5‐dependent stem elongation. Here we report the identification of a gene responsible for sac52‐d, another semi‐dominant suppressor mutant of acl5‐1. SAC52 encodes ribosomal protein L10 (RPL10A), which is highly conserved among eukaryotes and implicated in translational regulation. Transformation of acl5‐1 mutants with a genomic fragment containing the sac52‐d allele rescued the dwarf phenotype of acl5‐1. GUS reporter activity under the control of a SAC51 promoter with its upstream ORF was higher in acl5‐1 sac52‐d than in acl5‐1, suggesting that suppression of the acl5‐1 phenotype by sac52‐d is attributable, in part, to enhanced translation of certain transcripts including SAC51. We also found that a T‐DNA insertion allele of SAC52/RPL10A causes lethality in the female gametophyte.