Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin

Changing the subject: An efficient linear solid‐phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C‐terminal mod...

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Published inAngewandte Chemie International Edition Vol. 49; no. 52; pp. 10149 - 10153
Main Authors El Oualid, Farid, Merkx, Remco, Ekkebus, Reggy, Hameed, Dharjath S., Smit, Judith J., de Jong, Annemieke, Hilkmann, Henk, Sixma, Titia K., Ovaa, Huib
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 27.12.2010
WILEY‐VCH Verlag
Subjects
Amino Acid Sequence
chemical ligation
Communication
Molecular Sequence Data
Mutation
peptides
Peptides - chemical synthesis
Peptides - chemistry
Peptides - genetics
Protein Folding
solid-phase synthesis
synthetic methods
ubiquitin
Ubiquitin - chemical synthesis
Ubiquitin - chemistry
Ubiquitin - genetics
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Summary:Changing the subject: An efficient linear solid‐phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C‐terminal modification and the construction of all diubiquitin conjugates in high yields and purities in a straightforward manner.
Bibliography:Netherlands Foundation for Scientific Research
Funded Access
ark:/67375/WNG-5DPLNWK7-F
European Union
istex:8A45AE2CF0B35F2DF99EB25B27A0D9C37E2F5467
We thank Pim van Dijk for assistance with purification, Alex Faesen for expression of USP7/HAUSP and the UbAMC assays, Arjan Wiskerke (VU, Amsterdam) for assistance with CD measurements, Paul Geurink for experimental assistance, and Peter White (Novabiochem, Merck Chemicals) for useful suggestions. This research was sponsored by a grant from the Netherlands Foundation for Scientific Research (to H.O.), a European Union Marie Curie Reintegration Grant (to F.E.), EU Rubicon, KWF, and CBG grants to T.K.S.
ArticleID:ANIE201005995
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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We thank Pim van Dijk for assistance with purification, Alex Faesen for expression of USP7/HAUSP and the UbAMC assays, Arjan Wiskerke (VU, Amsterdam) for assistance with CD measurements, Paul Geurink for experimental assistance, and Peter White (Novabiochem, Merck Chemicals) for useful suggestions. This research was sponsored by a grant from the Netherlands Foundation for Scientific Research (to H.O.), a European Union Marie Curie Reintegration Grant (to F.E.), EU Rubicon, KWF, and CBG grants to T.K.S.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201005995