Coupling between skeletal muscle fiber size and capillarization is maintained during healthy aging

• Published in: Journal of cachexia, sarcopenia and muscle Vol. 8; no. 4; pp. 647 - 659
• Main Authors: Barnouin, Yoann, McPhee, Jamie S., Butler‐Browne, Gillian, Bosutti, Alessandra, De Vito, Giuseppe, Jones, David A., Narici, Marco, Behin, Anthony, Hogrel, Jean‐Yves, Degens, Hans
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.08.2017; Wiley Open Access/Springer Verlag; John Wiley and Sons Inc
• Subjects: Adult; Age; Aged; Ageing; Aging; Aging - physiology; Atrophy; Biopsy; Capillaries - cytology; Capillaries - pathology; Capillary; Cell Size; Female; Geriatry and gerontology; Healthy Aging - pathology; Healthy Aging - physiology; Histology; Human health and pathology; Humans; Life Sciences; Male; Metabolites; Mitochondrial DNA; Muscle Fibers, Skeletal - cytology; Muscle Fibers, Skeletal - pathology; Muscle fibre; Muscle, Skeletal - blood supply; Muscle, Skeletal - pathology; Original; Oxidative capacity; Oxygen Consumption - physiology; Sarcopenia; Studies; Succinate dehydrogenase; Tissues and Organs; Women; Young Adult; Young adults; United Kingdom > UK; France; Succinate dehydrogenase; Oxidative capacity; Muscle fibre; Ageing; Capillary
• ISSN: 2190-5991; 2190-6009; 2190-6009
• DOI: 10.1002/jcsm.12194

Background As muscle capillarization is related to the oxidative capacity of the muscle and the size of muscle fibres, capillary rarefaction may contribute to sarcopenia and functional impairment in older adults. Therefore, it is important to assess how ageing affects muscle capillarization and the interrelationship between fibre capillary supply with the oxidative capacity and size of the fibres. Methods Muscle biopsies from healthy recreationally active young (22 years; 14 men and 5 women) and older (74 years; 22 men and 6 women) people were assessed for muscle capillarization and the distribution of capillaries with the method of capillary domains. Oxidative capacity of muscle fibres was assessed with quantitative histochemistry for succinate dehydrogenase (SDH) activity. Results There was no significant age‐related reduction in muscle fibre oxidative capacity. Despite 18% type II fibre atrophy (P = 0.019) and 23% fewer capillaries per fibre (P < 0.002) in the old people, there was no significant difference in capillary distribution between young and old people, irrespective of sex. The capillary supply to a fibre was primarily determined by fibre size and only to a small extent by oxidative capacity, irrespective of age and sex. Based on SDH, the maximal oxygen consumption supported by a capillary did not differ significantly between young and old people. Conclusions The similar quantitative and qualitative distribution of capillaries within muscle from healthy recreationally active older people and young adults indicates that the age‐related capillary rarefaction, which does occur, nevertheless maintains the coupling between skeletal muscle fibre size and capillarization during healthy ageing.