季铵盐化纳米金刚石-肌红蛋白修饰电极的电化学行为

通过一系列的化学反应对纳米金刚石（ND）表面进行修饰,成功制备了季铵盐化纳米金刚石：ND-CO-NH-CH2-CH2-N（CH3）3^＋·I^-（QAS-ND）,通过FT-IR、元素分析、电化学等手段对目标产物QAS-ND进行了表征。将肌红蛋白（Mb）与QAS-ND混合液滴加在玻碳（GC）电极表面,制备QAS-ND/Mb/GC修饰电极。在0.1 mol/L磷酸盐缓冲溶液（pH 7.0）中,固定在膜内的Mb表现出良好的直接电化学性质,并显示了很好的稳定性。同时,探讨了此修饰电极表面固定的Mb对H2O2的催化还原,结果表明,此修饰电极可作为H2O2生物传感器,实现对H2O2的快速、准确检测,检出限...

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Published in分析化学 Vol. 42; no. 9; pp. 1332 - 1337
Main Author 马晓玲 丁承君 张璞 郭玮 罗红霞
Format Journal Article
LanguageChinese
Published 中国人民大学化学系,北京,100872 2014
Subjects
化学修饰电极
季铵盐化纳米金刚石
循环伏安法
肌红蛋白
过氧化氢
过氧化氢
Myoglobin
Cyclic voltammetry
肌红蛋白
Hydrogen peroxide
Quaternarized nanodiamond
循环伏安法
化学修饰电极
季铵盐化纳米金刚石
Chemically modified electrode
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ISSN0253-3820
DOI10.11895/j.issn.0253-3820.140093

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Summary:通过一系列的化学反应对纳米金刚石（ND）表面进行修饰,成功制备了季铵盐化纳米金刚石：ND-CO-NH-CH2-CH2-N（CH3）3^＋·I^-（QAS-ND）,通过FT-IR、元素分析、电化学等手段对目标产物QAS-ND进行了表征。将肌红蛋白（Mb）与QAS-ND混合液滴加在玻碳（GC）电极表面,制备QAS-ND/Mb/GC修饰电极。在0.1 mol/L磷酸盐缓冲溶液（pH 7.0）中,固定在膜内的Mb表现出良好的直接电化学性质,并显示了很好的稳定性。同时,探讨了此修饰电极表面固定的Mb对H2O2的催化还原,结果表明,此修饰电极可作为H2O2生物传感器,实现对H2O2的快速、准确检测,检出限为3.5μmol/L（S/N=3）。
Bibliography:MA Xiao-Ling, DING Cheng-Jun, ZHANG Pu, GUO Wei, LUO Hong-Xia （ Department of Chemistry, Renmin University of China, Beijing 100872, China）
22-1125/O6
Through a series of chemical reactions, a kind of quaternary ammonium salt derivative of nanodiamond, ND-CO-NH-CH2-CH2-N（CH3）3^＋·I^-（QAS-ND）, was obtained , which was confirmed by FTIR, element analysis experiment and the electrochemistry measurements. Mixed myoglobin （Mb） and QAS- ND solution was dropped on the surface of the glassy carbon （ GC ） electrode to prepare QAS-ND/Mb/GC modified electrode. In 0.1 mol/L phosphate buffer solution （PBS） （ pH 7.0）, Mb in the membrane exhibited direct electrochemical properties and showed good stability. The electrocatalytic property of the modified electrode toward H202 was investigated, the results showed that the modified electrode could be used as the H202 biosensor to achieve fast, accurate detection of H202, with a detection limit of 3.5 mmol/L （S/N=3）.
Quaternarized nanodiamond ; Myoglobin ; Chemically modified electr
ISSN:0253-3820
DOI:10.11895/j.issn.0253-3820.140093