10种中草药提取物体外抗铜绿假单胞菌作用研究

通过对粗糠柴等10种中草药采用80%乙醇室温下浸渍制备的提取物进行体外抗铜绿假单胞菌及其耐药菌活性研究,并采取药敏纸片法测定临床分离菌株的耐药性。结果表明：这10种中草药80%乙醇提取物中,粗糠柴的乙酸乙酯层对铜绿假单胞菌标准菌及其耐药菌的抑菌效果最好,其抑菌圈直径范围在10-17 mm之间,MIC范围在0.125-0.5 mg·mL（-1）之间,MBC范围在0.5-1 mg·mL（-1）之间;正丁醇层、水层的抑菌活性较乙酸乙酯层弱,石油醚层对铜绿假单胞菌没有效果。而小叶藤黄、滇南红厚壳、续随子的乙酸乙酯层,巴豆、罗汉松、肉桂醇提物对铜绿假单胞菌及其耐药菌株有较弱抗菌活性;滇南红厚壳的正丁醇层...

Full description

Saved in:
Bibliographic Details
Published in广西植物 Vol. 36; no. 2; pp. 240 - 245
Main Author 谢俊杰 韩峻 左国营 王宁 程子梦
Format Journal Article
LanguageChinese
Published 云南中医学院,昆明650500%云南中医学院,昆明,650500%成都军区昆明总医院 天然药物研究中心,昆明,650032 2016
成都军区昆明总医院 天然药物研究中心,昆明650032
Subjects
MIC/MBC
中草药
抗菌活性
粗糠柴
铜绿假单胞菌
antibacterial activity
Mallotus philippensi
中草药
Chinese herbal medicines
粗糠柴
铜绿假单胞菌
Pseudomonas aeruginosa
抗菌活性
MIC/MBC
Online AccessGet full text

Cover

More Information
Summary:通过对粗糠柴等10种中草药采用80%乙醇室温下浸渍制备的提取物进行体外抗铜绿假单胞菌及其耐药菌活性研究,并采取药敏纸片法测定临床分离菌株的耐药性。结果表明：这10种中草药80%乙醇提取物中,粗糠柴的乙酸乙酯层对铜绿假单胞菌标准菌及其耐药菌的抑菌效果最好,其抑菌圈直径范围在10-17 mm之间,MIC范围在0.125-0.5 mg·mL（-1）之间,MBC范围在0.5-1 mg·mL（-1）之间;正丁醇层、水层的抑菌活性较乙酸乙酯层弱,石油醚层对铜绿假单胞菌没有效果。而小叶藤黄、滇南红厚壳、续随子的乙酸乙酯层,巴豆、罗汉松、肉桂醇提物对铜绿假单胞菌及其耐药菌株有较弱抗菌活性;滇南红厚壳的正丁醇层、续随子乙酸乙酯层以及大八角和郁金的醇提物对铜绿假单胞菌及其耐药菌株均无活性。从这些数据中可以得出,粗糠柴的乙酸乙酯层、正丁醇层和水层对铜绿假单胞菌及其耐药菌有较好的抑菌活性,尤以乙酸乙酯层活性最好,而粗糠柴的石油醚层没有活性。
Bibliography:Chinese herbal medicines; Mallotus philippensi; Pseudomonas aeruginosa; MIC/MBC; antibacterial activity
XIE Jun-Jie,HAN Jun,ZUO Guo-Ying,WANG Ning,CHENG Zi-Meng（.Research Center for Natural Medicines, Kunming General Hospital of Chengdu Military Command, Kunming650032, China; 2. Yunnan Traditional Chinese Medical University, Kunming 650500, China ）
45-1134/Q
To investigate the in vitro antibacterial activities of 80%ethanol extracts from 10 Chinese herbal medicines against Pseudomonas aeruginosa and its resistant strains,the drug-resistance spectrum of 7P.aeruginosa stains isolated from the clinical sputum samples was determined by the Kirby-Bauer（K-B） method.The dried powder of the collected 10 Chinese herbal medicine samples were extracted with 80%ethanol and the solvent was evaporated under reduced pressure to get the Chinese herbal medicines ethanol extracts.The ethanol extracts were suspended in deioned water,then petroleum ether,ethyl acetate,and n-BuOH were used to extract successively.The activities of e
ISSN:1000-3142
DOI:10.11931/guihaia.gxzw201502015