利用ISSR分子标记检测空间诱导罗汉果DNA突变

为探究太空环境对罗汉果造成的诱变效应,筛选罗汉果新品种培育优异种质,该研究运用ISSR分子标记技术,对28个航天诱变罗汉果及主栽品种进行了全基因组多态性检测和聚类分析。结果表明：从100个ISSR引物中筛选得到17个引物,共扩增出157个条带,其中83条具有多态性,多态条带百分率为52.87%,遗传相似系数范围为0.707~0.987。根据UPGMA聚类图,28个罗汉果样本可以分为3类：聚类Ⅰ为航天种质B6和B3♀;聚类Ⅱ为航天种质A1♀、A14、A18♂与主栽品种;聚类Ⅲ中都为航天罗汉果种质。上述结果暗示A1♀、A14、A18♂与其他航天种质已经产生了一定的遗传分化,具有与主栽品种相似的遗传...

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Bibliographic Details
Published in广西植物 Vol. 37; no. 5; pp. 581 - 586
Main Author 向巧彦 黄夕洋 李虹 甘金佳 梁勇诗 蒋水元
Format Journal Article
LanguageChinese
Published 广西壮族自治区中国科学院 广西植物研究所,广西 桂林,541006 2017
Subjects
ISSR
分子标记辅助选择
空间诱变育种
罗汉果
罗汉果
molecular marker-assisted selection
空间诱变育种
分子标记辅助选择
space mutation breeding
ISSR
Siraitia grosvenorii
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Summary:为探究太空环境对罗汉果造成的诱变效应,筛选罗汉果新品种培育优异种质,该研究运用ISSR分子标记技术,对28个航天诱变罗汉果及主栽品种进行了全基因组多态性检测和聚类分析。结果表明：从100个ISSR引物中筛选得到17个引物,共扩增出157个条带,其中83条具有多态性,多态条带百分率为52.87%,遗传相似系数范围为0.707~0.987。根据UPGMA聚类图,28个罗汉果样本可以分为3类：聚类Ⅰ为航天种质B6和B3♀;聚类Ⅱ为航天种质A1♀、A14、A18♂与主栽品种;聚类Ⅲ中都为航天罗汉果种质。上述结果暗示A1♀、A14、A18♂与其他航天种质已经产生了一定的遗传分化,具有与主栽品种相似的遗传背景,可能获得了有益突变。该研究结果为罗汉果新品种培育和杂交亲本选配提供了科学依据。
Bibliography:XIANG Qiao-Yan, HUANG Xi-Yang, LI Hong, GAN Jin-Jia, LIANG Yong-Shi, JIANG Shui-Yuan （Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China ）
45-1134/Q
Siraitia grosvenorii, space mutation breeding, ISSR, molecular marker-assisted selection
In order to investigate genetic variation of Siraitia grosvenorii after space flight, and to create elite germplasms, ISSR（inter-simple sequence repeats）molecular markers were used to detect the genome-wide DNA polymorphism and to make cluster analysis of 28 S. grosvenorii samples including space-flight accessions and main cultivars. Se-venteen primers which had clear polymorphic bands were selected from 100 primers. A total of 157 bands were detected, of which 83 bands were polymorphic with a polymorphic rate of 52.87%. Genetic similarity coefficient ranged from 0.707 to 0.987. UPGMA cluster analysis revealed that 28 samples formed three primary distinct clusters： Cluster I included two space mutation ger
ISSN:1000-3142
DOI:10.11931/guihaia.gxzw201604022