A preliminary study of imaging paclitaxel-induced tumor apoptosis with 99Tcm-His10-Annexin V

• Published in: 中华医学杂志（英文版） Vol. 126; no. 15; pp. 2928 - 2933
• Main Author: ZHENG Yu-min WANG Feng FANG Wei HUA Zi-chun WANG Zi-zheng MENG Qing-le YAN Jue
• Format: Journal Article
• Language: English
• Published: Department of Nuclear Medicine, China-Japan Friendship Hospital,Beijing 100029, China%Department of Nuclear Medicine, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing, Jiangsu 210006, China%Department of Nuclear Medicine, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037,China%The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, China 2013
• Subjects: Annexin; caspase-3; 成像; 标记法; 紫杉醇; 肿瘤细胞凋亡; 诱导; 非小细胞肺癌; non-small cell lung cancer; apoptosis; His10-Annexin V
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20122008

Backgroud in tumors the process of apoptosis occurs over an interval of time after chemotherapy, it is important to determine the best time for detecting apoptosis by in vivo imaging. In this study, we evaluated the dynamics and feasibility of imaging non-small cell lung cancer （NSCLC） apoptosis induced by paclitaxel treatment using a 99Tcm-labeled Annexin V recombinant with ten consecutive histidines （Hislo-Annexin V） in a mouse model. Methods 99Tcm-His10-Annexin V was prepared by one step direct labeling; radio-chemical purity （RCP） and radio-stability was tested. The binding of 99Tcm-His10-Annexin V to apoptotic cells was validated in vitro using camptothecin-induced Jurkat cells. In vivo bio-distribution was determined in mice by dissection. The human H460 NSCLC tumor cell line （H460） tumor-bearing mice were treated with intravenous paclitaxel 24, 48 and 72 hours later. 99Tcm-His10-Annexin V was injected intravenously, and planar images were acquired at 2, 4 and 6 hours post-injection on a dual-head gamma camera fitted with a pinhole collimator. Tumor-to-normal tissue ratios （T/NT） were calculated by ROI analysis and they reflected specific binding of 99Tcm-His10-Annexin V. Mice were sacrificed after imaging. Caspase-3, as the apoptosis detector, was determined by flow cytometry, and DNA fragmentation was analyzed by the terminal deoxynucleotidytransferase mediated dUTP nick-end labeling （TUNEL） assay. Nonspecific accumulation of protein was estimated using bovine serum albumin （BSA）. The imaging data were correlated with TUNEL-positive nuclei and caspase-3 activity. Results 99Tcm-His10-Annexin V had a RCP 〉98% and high stability 2 hours after radio-labeling, and it could bind to apoptotic cells with high affinity. Bio-distribution of 99Tcm-His10-Annexin V showed predominant uptake in kidney, relatively low uptake in myocardium, liver and gastrointestinal tract, and rapid clearance from blood and kidney was observed. The TINT was significantly increased after paclitaxel treatment, whereas it was low in untreated tumors （T/NT=1.43±0.18）. The %lD/g activity in Group 2 （24 hours）, Group 3 （48 hours） and Group 4 （72 hours） after treatment was 2.55±0.73, 3.35±1.10, and 3.4±0.96, respectively. Whereas in the non-treated group, Group 1, %lD/g was 1.10±0.18. The radiotracer uptake was positively correlated to the apoptotic index （r=0.852, P〈0.01）, as well as caspase-3 activity （r=0.816, P〈0.01）. Conclusion This study addresses the dynamics and feasibility of imaging non-small cell lung tumor apoptosis using 99Tcm- His10-Annexin V.