莲心碱促进膀胱癌5637细胞凋亡增强Caspase-7表达及活化

目的研究莲心碱（Liensinine）对膀胱癌细胞株5637凋亡的影响,初步探讨其作用机制。方法使用CCK-8法及克隆形成实验检测莲心碱对5637细胞增殖的抑制作用;通过流式细胞术Annexin V-FITC/PI双染法测定莲心碱对5637细胞凋亡的影响;采用Western blot技术分析细胞凋亡相关基因Caspase-7表达状况。结果 CCK-8法及克隆形成实验结果均表明莲心碱对膀胱癌细胞5637增殖有明显的抑制作用,并呈剂量-效应关系。流式细胞术结果提示莲心碱可使膀胱癌5637细胞发生早期凋亡;Western blot检测结果显示莲心碱增强5637细胞Caspase-7表达并促进其活化。...

Full description

Saved in:
Bibliographic Details
Published in天津医药 Vol. 43; no. 7; pp. 724 - 727
Main Author 徐宾 张天禹 谭宁 闫亓 徐伶俐 刘晓佳 高漓
Format Journal Article
LanguageChinese
Published 桂林医学院附属医院泌尿外科 邮编541004%桂林医学院科学实验中心%桂林医学院附属医院消化内科%桂林医学院附属医院病理科 2015
Subjects
Caspase-7
半胱氨酸内肽酶类
生物碱类
细胞凋亡
细胞增殖
膀胱肿瘤
莲心碱
细胞凋亡
半胱氨酸内肽酶类
膀胱肿瘤
cell proliferation
alkaloids
liensinine
apoptosis
生物碱类
Caspase-7
cysteine endopeptidases
urinary bladder neoplasms
莲心碱
细胞增殖
Online AccessGet full text
ISSN0253-9896
DOI10.11958/j.issn.0253-9896.2015.07.006

Cover

More Information
Summary:目的研究莲心碱（Liensinine）对膀胱癌细胞株5637凋亡的影响,初步探讨其作用机制。方法使用CCK-8法及克隆形成实验检测莲心碱对5637细胞增殖的抑制作用;通过流式细胞术Annexin V-FITC/PI双染法测定莲心碱对5637细胞凋亡的影响;采用Western blot技术分析细胞凋亡相关基因Caspase-7表达状况。结果 CCK-8法及克隆形成实验结果均表明莲心碱对膀胱癌细胞5637增殖有明显的抑制作用,并呈剂量-效应关系。流式细胞术结果提示莲心碱可使膀胱癌5637细胞发生早期凋亡;Western blot检测结果显示莲心碱增强5637细胞Caspase-7表达并促进其活化。结论莲心碱可抑制5637细胞增殖,使其发生早期凋亡,其作用机制可能与促进Caspase-7表达及活化有关。
Bibliography:Objective To study the effects of Liensinine on apoptosis of 5637 cells, and its mechanism thereof. Methods CCK-8 method and the colony formation test were used to detect cell viabilities, and then inhibition rates were calcu-lated. Flow cytometry was used to detect the effects of Liensinine on apoptosis of 5637 cells. Western blot assay was used todetect Caspase-7 protein expression. Results CCK-8 assay and colony formation test indicated that Liensinine inhibitedthe cell proliferation significantly. Results of flow cytometry indicated that Liensinine induced early apoptosis of 5637 cells.Western blot assay showed that Liensinine improved the expression of Caspase-7 and enhanced the activation of Caspase-7in 5637 cells. Conclusion Liensinine could inhibit the proliferation of 5637 cells, induce early apoptosis, which may be re-lated with the enhanced expression of Caspase-7 and its activation.
XU Bin, ZHANG Tianyu, TAN Ning, YAN Qi , xu Lingli, LIU Xiaojia, GAO Li（ 1 Department of Urology, Affiliated Hospital
ISSN:0253-9896
DOI:10.11958/j.issn.0253-9896.2015.07.006