Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species

• Published in: BMC genomics Vol. 24; no. 1; pp. 1 - 667
• Main Authors: Ahmed, Noha A, Khattab, Rania Abdelmonem, Ragab, Yasser M, Hassan, Mariam
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 06.11.2023; BioMed Central; BMC
• Subjects: Acids; Anopheles; Antibacterial activity; Antibacterial agents; Antibiotic resistance; Antibiotics; Bile; Carbon dioxide; Cellulose acetate; Chloramphenicol; Chloromycetin; Comparative analysis; CRISPR-cas; Dietary supplements; Drug resistance; Drug resistance in microorganisms; E coli; Enterococcus; Enterococcus faecium; Escherichia coli; Ethylenediaminetetraacetic acid; Genes; Genetic analysis; Genomes; Genomics; Glycerol; Gut microbiota; Human stool; Health aspects; Intestinal microflora; Methods; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Nosocomial infection; Probiotics; Safety; Species; Tetracycline; Tetracyclines; Transposons; Vancomycin; Virulence; Virulence (Microbiology); Whole genome sequencing; United Kingdom; United Kingdom > UK; United States > US; Germany
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-023-09749-9

The gut microbiota is considered a rich source for potential novel probiotics. Enterococcus genus is a normal component of a healthy gut microbiota, suggesting its vital role. Nosocomial infections caused mainly by E. facalis and E. faecium have been attributed to the plasticity of the Enterococcus genomes. In this study, we assessed the probiotic and safety characteristics of two E. lactis strains isolated from the human gut microbiota using in-vitro and in silico approaches. Additionally, the safety of the E. lactis species was evaluated using comparative genomics analysis. The two E. lactis strains 10NA and 50NA showed resistance to bile salts and acid tolerance with antibacterial activity against Escherichia coli, Salmonella typhi, and Clostridioides difficile. For safety assays, the two strains did not display any type of hemolysis on blood agar, and the survival of Caco-2 cells was not significantly different (P-value > 0.05) compared to the control using cell free supernatants at 100% (v/v), 50% (v/v), 10% (v/v), and 5% (v/v) concentrations. Regarding antibiotic susceptibility, both strains were sensitive to vancomycin, tetracycline, and chloramphenicol. Comprehensive whole-genome analysis revealed no concerning associations between virulence or antibiotic resistance genes and any of the identified mobile genetic elements. Comparative genome analysis with closely related E. faecium species genomes revealed the distinctive genomic safety of the E. lactis species. Our two E. lactis strains showed promising probiotic properties in-vitro. Their genomes were devoid of any transferable antibiotic resistance genes. In silico comparative analysis confirmed the safety of the E. lactis species. These results suggest that E. lactis species could be a potential source for safer Enterococcus probiotic supplements.