Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 83; no. 19; pp. 7344 - 7347
• Main Authors: Revuelta, J.L, Jayaram, M
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.10.1986; National Acad Sciences
• Subjects: Biological and medical sciences; Biotechnology; Centrifugation; CHAMPIGNON; DNA; DNA replication; Drug Resistance, Microbial; Fundamental and applied biological sciences. Psychology; FUNGI; Genes; Genetic engineering; Genetic technics; GENETIC TRANSFORMATION; Genetic Vectors; GENETICA; GENETICS; GENETIQUE; Gentamicins - pharmacology; Methods. Procedures. Technologies; Mucorales - genetics; Mycelium; Phycomyces; Phycomyces - genetics; PLASMIDE; PLASMIDIOS; PLASMIDS; Replicon; Room temperature; Saccharomyces cerevisiae - genetics; Spheroplasts; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Transformation, Genetic; Yeasts; Plasmid; Phycomyces blakesleeanus; Genetic transformation; Phycomycetes; Thallophyta
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.83.19.7344

The fungus, Phycomyces blakesleeanus, shows many well-defined responses to a number of external stimuli. Genetic analysis shows that at least eight genes are involved in Phycomyces sensory transduction. As a first step toward the molecular analysis of these genes and their products, we have developed a transformation protocol for Phycomyces by using a plasmid containing the kanamycinresistance gene from Tn903 and a Phycomyces DNA fragment capable of supporting autonomous replication in yeast (ARS). Our results demonstrate that the Tn903 gene is expressed in Phycomyces and that the ARS fragment selected in yeast supports autonomous replication in Phycomyces as well.