Functional relationships between plasmids and their significance for metabolism and symbiotic performance of Rhizobium leguminosarum bv. trifolii

• Published in: Journal of applied genetics Vol. 55; no. 4; pp. 515 - 527
• Main Authors: Stasiak, Grażyna, Mazur, Andrzej, Wielbo, Jerzy, Marczak, Małgorzata, Żebracki, Kamil, Koper, Piotr, Skorupska, Anna
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2014; Springer
• Subjects: Analysis; Animal Genetics and Genomics; Bacteria; Beans; Biomedical and Life Sciences; Cell Movement - physiology; DNA, Bacterial - genetics; Genes, Bacterial - genetics; Genomics; Human Genetics; Legumes; Life Sciences; Microbial Genetics and Genomics; Microbial Genetics • Original Paper; Mimosaceae; Physiological aspects; Plant Genetics and Genomics; Plasmids - genetics; Polysaccharides; Polysaccharides, Bacterial - metabolism; Rhizobium leguminosarum; Rhizobium leguminosarum - genetics; Rhizobium leguminosarum - growth & development; Rhizobium leguminosarum - metabolism; Seeds - microbiology; Soil microbiology; Symbiosis - physiology; Tetracycline; Tetracyclines; Transposons; Trifolium - genetics; Trifolium - microbiology; Symbiosis; Plasmid curing; Metabolism; Megaplasmids
• ISSN: 1234-1983; 2190-3883
• DOI: 10.1007/s13353-014-0220-2

Rhizobium leguminosarum bv. trifolii TA1 (RtTA1) is a soil bacterium establishing a highly specific symbiotic relationship with clover, which is based on the exchange of molecular signals between the host plant and the microsymbiont. The RtTA1 genome is large and multipartite, composed of a chromosome and four plasmids, which comprise approximately 65 % and 35 % of the total genome, respectively. Extrachromosomal replicons were previously shown to confer significant metabolic versatility to bacteria, which is important for their adaptation in the soil and nodulation competitiveness. To investigate the contribution of individual RtTA1 plasmids to the overall cell phenotype, metabolic properties and symbiotic performance, a transposon-based elimination strategy was employed. RtTA1 derivatives cured of pRleTA1b or pRleTA1d and deleted in pRleTA1a were obtained. In contrast to the in silico predictions of pRleTA1b and pRleTA1d, which were described as chromid-like replicons, both appeared to be completely curable. On the other hand, for pRleTA1a (symbiotic plasmid) and pRleTA1c, which were proposed to be unessential for RtTA1 viability, it was not possible to eliminate them at all (pRleTA1c) or entirely (pRleTA1a). Analyses of the phenotypic traits of the RtTA1 derivatives obtained revealed the functional significance of individual plasmids and their indispensability for growth, certain metabolic pathways, production of surface polysaccharides, autoaggregation, biofilm formation, motility and symbiotic performance. Moreover, the results allow us to suggest broad functional cooperation among the plasmids in shaping the phenotypic properties and symbiotic capabilities of rhizobia.