Antitumor 1, 3-β-Glucan from Cultured Fruit Body of Sparassis crispa

• Published in: Biological & pharmaceutical bulletin Vol. 23; no. 7; pp. 866 - 872
• Main Authors: OHNO, Naohito, MIURA, Noriko N., NAKAJIMA, Mitsuhiro, YADOMAE, Toshiro
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 2000; Maruzen
• Subjects: Animals; Antineoplastic agents; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - therapeutic use; antitumor activity; beta-Glucans; Biological and medical sciences; Disease Models, Animal; Drug Screening Assays, Antitumor; General aspects; General pharmacology; Glucans - chemistry; Glucans - isolation & purification; Glucans - therapeutic use; hematopoiesis; immunomodulation; Leukocyte Count; Leukopenia - chemically induced; Magnetic Resonance Spectroscopy; Male; Medical sciences; Mice; Mice, Inbred ICR; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Polyporales - chemistry; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Sarcoma 180 - drug therapy; Sparassis crispa; Structure-Activity Relationship; β-glucan; Antineoplastic agent; Solid tumor; Intraperitoneal administration; Pharmacognosy; Rodentia; Oral administration; Basidiomycetes; Primary structure; Malignant tumor; Biological activity; Fungi; Glucan; Sparassis crispa; Vertebrata; Mammalia; Structure activity relation; Mouse; Animal; Thallophyta
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.23.866

Sparassis crispa is an edible mushroom recently cultivable in Japan. Polysaccharide fractions were prepared from the cultured S. crispa by repeated extraction with hot water (SCHWE), cold NaOH (SCCA), and then hot NaOH (SCHA). HWE was further separated by 1 volume (SCHWE1v) or 4 volumes (SCHWE4v) of ethanol-precipitable fractions. By chemical, enzymic, and NMR analyses, the primary structures of SCHWE1v, SCCA, and SCHA were 6-branched 1, 3-β-glucan, having one branch in approximately every third mainchain unit. All of these fractions showed antitumor activity to the solid form of Sarcoma 180 in ICR mice with strong vascular dilation and hemorrhage reaction. These fractions also showed enhanced hematopoietic response to cyclophosphamide induced leukopenic mice following intraperitoneal or peroral administration.