Antimicrobial activity of Calendula officinalis, Camellia sinensis and chlorhexidine against the adherence of microorganisms to sutures after extraction of unerupted third molars

• Published in: Journal of applied oral science Vol. 19; no. 5; pp. 476 - 482
• Main Authors: Faria, Raquel Lourdes, Cardoso, Lincoln Marcelo Lourenço, Akisue, Gokithi, Pereira, Cristiane Aparecida, Junqueira, Juliana Campos, Jorge, Antonio Olavo Cardoso, Santos Júnior, Paulo Villela
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01.10.2011; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Adolescent; Adult; Anti-Infective Agents - pharmacology; Antimicrobial activity; Bacterial Adhesion - drug effects; Calendula; Calendula officinalis L; Camellia sinensis; Camellia sinensis (L.) Kuntze; Candida - drug effects; Chlorhexidine; Chlorhexidine - pharmacology; Colony Count, Microbial; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Humans; Molar, Third - surgery; Mouthwashes - pharmacology; Original; Phytotherapy - methods; Plant Extracts - pharmacology; Sutures - microbiology; Time Factors; Tooth Extraction; Young Adult; Antimicrobial activity; Chlorhexidine; Camellia sinensis (L.) Kuntze; Calendula officinalis L
• ISSN: 1678-7757; 1678-7765; 1678-7765; 1678-7757
• DOI: 10.1590/S1678-77572011000500007

The objective of this study was to compare the antimicrobial effect of mouthwashes containing Calendula officinalis L., Camellia sinensis (L.) Kuntze and 0.12% chlorhexidine digluconate on the adherence of microorganisms to suture materials after extraction of unerupted third molars. Eighteen patients with unerupted maxillary third molars indicated for extraction were selected (n=6 per mouthwash). First, the patients were subjected to extraction of the left tooth and instructed not to use any type of antiseptic solution at the site of surgery (control group). After 15 days, the right tooth was extracted and the patients were instructed to use the Calendula officinalis, Camellia sinensis or chlorhexidine mouthwash during 1 week (experimental group). For each surgery, the sutures were removed on postoperative day 7 and placed in sterile phosphate-buffered saline. Next, serial dilutions were prepared and seeded onto different culture media for the growth of the following microorganisms: blood agar for total microorganism growth; Mitis Salivarius bacitracin sucrose agar for mutans group streptococci; mannitol agar for Staphylococcus spp.; MacConkey agar for enterobacteria and Pseudomonas spp., and Sabouraud dextrose agar containing chloramphenicol for Candida spp. The plates were incubated during 24-48 h at 37ºC for microorganism count (CFU/mL). The three mouthwashes tested reduced the number of microorganisms adhered to the sutures compared to the control group. However, significant differences between the control and experimental groups were only observed for the mouthwash containing 0.12% chlorhexidine digluconate. Calendula officinalis L. and Camellia sinensis (L.) Kuntze presented antimicrobial activity against the adherence of microorganisms to sutures but were not as efficient as chlorhexidine digluconate.