Genomic and probiotic characterization of SJP-SNU strain of Pichia kudriavzevii

• Published in: AMB Express Vol. 8; no. 1; pp. 80 - 9
• Main Authors: Hong, Seung-Min, Kwon, Hyuk-Joon, Park, Se-Joon, Seong, Won-Jin, Kim, Ilhwan, Kim, Jae-Hong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 17.05.2018; Springer Nature B.V; SpringerOpen
• Subjects: Amino acid sequence; Bile; Biomedical and Life Sciences; Biotechnology; Cecum; Cellulase; Chickens; Comparative genomics; De novo sequencing; Deoxyribonucleic acid; DNA; Embryos; Evolution; Gene sequencing; Genes; Genomic analysis; Hydrogen sulfide; Intestine; Life Sciences; Microbial Genetics and Genomics; Microbiology; Mitochondrial DNA; Novel yeast; Nucleotide sequence; Original; Original Article; Pathogenicity; Pichia kudriavzevii; Probiotics; Salmonella; Yeast; Pathogenicity; Comparative genomics; Probiotics; Evolution; De novo sequencing; Novel yeast
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-018-0609-0

The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces. SJP-SNU did not cause mortality in 10-day-old chicken embryos. From 13 initial contigs, 11 were finally assembled and represented 10 chromosomal sequences and 1 mitochondrial DNA sequence. Comparative genomic analyses revealed that SJP-SNU was a strain of Pichia kudriavzevii . Although SJP-SNU possesses pathogenicity-related genes, they showed very low amino acid sequence identities to those of Candida albicans . Furthermore, SJP-SNU possessed useful genes, such as phytases and cellulase. Thus, SJP-SNU is a useful yeast possessing the basic traits of a probiotic, and further studies to demonstrate its efficacy as a probiotic in the future may be warranted.