Investigation of milk microbiota of healthy and mastitic Sahiwal cattle

• Published in: BMC microbiology Vol. 23; no. 1; pp. 1 - 13
• Main Authors: Salman, Mian Muhammad, Nawaz, Muhammad, Yaqub, Tahir, Mushtaq, Muhammad Hassan
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 24.10.2023; BioMed Central; BMC
• Subjects: 16S rRNA; Akkermansia muciniphila; Analysis; Animals; Antibiotics; Bacteria; Bioinformatics; Biological diversity; Cattle; Diversity indices; Dominant species; Firmicutes; Households; Indigenous animals; Inflammation; International economic relations; Investigations; Lactobacillus fermentum; Livestock industry; Mastitis; Metagenomics; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Milk; Milk microbiota; Milk production; Phylogenetics; Probiotics; Proteobacteria; RNA; rRNA 16S; Sahiwal cattle; Software; Species diversity; Variance analysis; United States > US; California
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-023-03051-0

Sahiwal cattle is an indigenous cattle breed of Pakistan and mastitis is one of the major problems faced by Sahiwal cattle which hinders its production potential. The study was designed to investigate the milk microbiota of healthy and mastitic Sahiwal cattle as part of a multistep project to develop probiotics for the mitigation and control of mastitis. Milk samples of Sahiwal cattle (healthy clinical mastitis and subclinical mastitis) reared under similar husbandry and management practices were processed for 16S rRNA gene base metagenomics analysis. It is concluded that the milk microbiota of healthy sahiwal cattle has higher diversity and dominant taxa in the different groups may be used as signature microbes for mastitis susceptibility. Akkermansia muciniphila is one of candidate specie that was identified and may be used for development of probiotics.