Cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis

• Published in: Journal of Bacteriology Vol. 175; no. 20; pp. 6403 - 6410
• Main Authors: Nagata, Y, Nariya, T, Ohtomo, R, Fukuda, M, Yano, K, Takagi, M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1993
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Amino Acid Sequence; Bacterial Proteins - genetics; Base Sequence; BIODEGRADACION; BIODEGRADATION; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; CLONACION; CLONAGE; Cloning, Molecular; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Bacterial; Genetics; HCH; Hexachlorocyclohexane - metabolism; Hydrolases - genetics; LIASAS; LYASE; METABOLITE; METABOLITOS; Mission oriented research; Molecular Sequence Data; PSEUDOMONAS; Pseudomonas - enzymology; Pseudomonas - genetics; Pseudomonas paucimobilis; SECUENCIA NUCLEICA; Sequence Alignment; Sequence Deletion; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; Pseudomonadales; Biodegradation; Insecticide; Dehalogenation; Nucleotide sequence; Enzyme; Metabolism; Pseudomonas; Dechlorination; Organochlorine compounds; Bacteria; Pseudomonadaceae; Molecular cloning; Aminoacid sequence
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.175.20.6403-6410.1993

In Pseudomonas paucimobilis UT26, gamma-hexachlorocyclohexane (gamma-HCH) is converted by two steps of dehydrochlorination to a chemically unstable dehalogenases, were good substrates for LinB, suggesting that LinB may be a member of haloalkane dehalogenases with broad-range specificity for substrates