Differential expression of CaMKII isoforms and overall kinase activity in rat dorsal root ganglia after injury

• Published in: Neuroscience Vol. 300; pp. 116 - 127
• Main Authors: Bangaru, M.L.Y, Meng, J, Kaiser, D.J, Yu, H, Fischer, G, Hogan, Q.H, Hudmon, A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 06.08.2015
• Subjects: Animals; Axotomy; Ca2+ signaling; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; CaMKII; Catalysis; Disease Models, Animal; Ganglia, Spinal - enzymology; Gene Expression; HEK293 Cells; Humans; Hyperalgesia - enzymology; Hyperalgesia - etiology; Isoenzymes - metabolism; Lumbar Vertebrae; Male; nerve injury; Neurology; neuropathic pain; Rats, Sprague-Dawley; RNA, Messenger - metabolism; sensory neuron; Sensory Receptor Cells - enzymology; Spinal Nerves - injuries; radioimmunoprecipitation assay; CaMKII-ir; CNS; mRNA; Ca 2+/calmodulin-dependent protein kinase II; phosphate-buffered saline; peripheral nervous system; RIPA; AC2; SNL; BSA; sensory neuron; analysis of variance; PNS; ANOVA; ethylenediaminetetraacetic acid; nerve injury; messenger ribonucleic acid; qPCR; glyceraldehyde-3-phosphate dehydrogenase; MAPK6; PBS; spinal nerve ligation; quantitative polymerase chain reaction; EDTA; Ca 2+ signaling; central nervous system; EGTA; neuropathic pain; autocamtide-2 peptide; CaMKII; mitogen-activated protein kinase 6; adenosine triphosphate; dorsal root ganglion; DRG; ethylene glycol tetraacetic acid; ATP; GAPDH; bovine serum albumin; CaMKII immunoreactivity; Ca2+ signaling; Ca(2+) signaling
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2015.05.007

Highlights • DRG neurons express mRNA for all CaMKII isoforms in the order of γ > α > β = δ. • Painful nerve injury reduces γ and α CaMKII in axotomized neurons, but elevates δ. • Neurons adjacent to axotomized neurons show depressed αCaMKII mRNA. • Total CaMKII protein and activity fall in axotomized more than in adjacent neurons. • Ca2+ -independent catalytic activity falls only in axotomized sensory neurons.