Removal of chronic Mycoplasma ovipneumoniae carrier ewes eliminates pneumonia in a bighorn sheep population

• Published in: Ecology and evolution Vol. 10; no. 7; pp. 3491 - 3502
• Main Authors: Garwood, Tyler J., Lehman, Chadwick P., Walsh, Daniel P., Cassirer, E. Frances, Besser, Thomas E., Jenks, Jonathan A.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.04.2020; John Wiley and Sons Inc; Wiley
• Subjects: Adults; Age; Animal populations; bighorn sheep; chronic carriage; Deoxyribonucleic acid; Disease; DNA; Enzymes; Epidemics; Health services; Hypotheses; Mortality; Mycoplasma ovipneumoniae; Original Research; Ovis canadensis; Parks & recreation areas; pathogen persistence; Pathogens; Pneumonia; Polymerase chain reaction; Population; Populations; Predictive control; Reduction; Respiratory diseases; RNA polymerase; Sheep; Statistical analysis; targeted removal; wildlife disease; South Dakota; United States > US; bighorn sheep; targeted removal; wildlife disease; pneumonia; chronic carriage; Mycoplasma ovipneumoniae; pathogen persistence
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.6146

Chronic pathogen carriage is one mechanism that allows diseases to persist in populations. We hypothesized that persistent or recurrent pneumonia in bighorn sheep (Ovis canadensis) populations may be caused by chronic carriers of Mycoplasma ovipneumoniae (Mo). Our experimental approach allowed us to address a conservation need while investigating the role of chronic carriage in disease persistence. We tested our hypothesis in two bighorn sheep populations in South Dakota, USA. We identified and removed Mo chronic carriers from the Custer State Park (treatment) population. Simultaneously, we identified carriers but did not remove them from the Rapid City population (control). We predicted removal would result in decreased pneumonia, mortality, and Mo prevalence. Both population ranges had similar habitat and predator communities but were sufficiently isolated to preclude intermixing. We classified chronic carriers as adults that consistently tested positive for Mo carriage over a 20‐month sampling period (n = 2 in the treatment population; n = 2 in control population). We failed to detect Mo or pneumonia in the treatment population after chronic carrier removal, while both remained in the control. Mortality hazard for lambs was reduced by 72% in the treatment population relative to the control (CI = 36%, 91%). There was also a 41% reduction in adult mortality hazard attributable to the treatment, although this was not statistically significant (CI = 82% reduction, 34% increase). Synthesis and Applications: These results support the hypothesis that Mo is a primary causative agent of persistent or recurrent respiratory disease in bighorn sheep populations and can be maintained by a few chronic carriers. Our findings provide direction for future research and management actions aimed at controlling pneumonia in wild sheep and may apply to other diseases. In this experimental study, we removed chronic shedders of Mycoplasma ovipneumoniae from a pneumonic bighorn sheep population and followed adult and juvenile survival in both the population and a nearby control population. The treatment population had significantly higher survival across age classes and no pneumonia or M. ovipneumoniae, whereas the control population suffered high mortality rates due to pneumonia. Pneumonia mortality is a widespread and persistent threat to bighorn sheep populations, and this is the first management action that has successfully removed pneumonia from a herd.