Mineral nutrient imbalance,DNA lesion and DNA-protein crosslink involved in growth retardation of Vicia faba L.seedlings exposed to lanthanum ions

• Published in: 环境科学学报（英文版） Vol. 24; no. 2; pp. 214 - 220
• Main Author: Chengrun Wang Kegui Zhang Mei He Chuanjun Jiang Liumin Tian Yuan Tian XiaorongWang
• Format: Journal Article
• Language: English
• Published: School of Life Science, Huainan Normal University, Huainan 232001, China%Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, USA%State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210093, China 2012
• Subjects: DNA损伤; 十二烷基硫酸钠; 单细胞凝胶电泳; 矿质营养元素; 营养失衡; 蚕豆; 蛋白质交联; 镧离子; DNA-protein crosslinks; mineral nutrient; single cell gel electrophoresis; rare earth elements; DNA lesion
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(11)60760-2

Effects of mineral nutrient imbalance,DNA lesion and DNA-protein crosslink on growth of Vicia faba L.seedlings hydroponically cultivated in concentrations of extraneous lanthanum（La） for 20 days were investigated in the present experiment.The results showed that contents of La,Cu or K elements in roots generally changed synchronously with those in leaves,while Ca,Fe,Zn,Mg,Mn or P in the roots altered inversely to those in the leaves.Thus,the extraneous La led to redistribution and imbalance of mineral nutrient elements in the roots and leaves.DNA lesion and DNA-protein crosslink were investigated by single cell gel electrophoresis（SCGE） and sodium dodecyl sulfate/potassium（SDS/K＋） precipitation methods,respectively.The results demonstrated that the increasing La induced DNA break and DNA-protein crosslinks（DPCs） in the seedlings.These results suggested that mineral nutrient imbalance,DNA lesion and DNA-protein crosslink were involved in the growth retardation and growth alteration of the seedlings,which may help to understand the mechanisms of rare earth elements（REEs） on plant growth.