Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

• Published in: Microbial cell factories Vol. 19; no. 1; pp. 1 - 212
• Main Authors: Li, Sihan, He, Youcheng, Zhang, Haiou, Zheng, Rong, Xu, Ruoying, Liu, Qihong, Tang, Shuihua, Ke, Xiao, Huang, Minghan
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 18.11.2020; BioMed Central; BMC
• Subjects: Abundance; Bacteria; Care and treatment; Chinese medicine; Constipation; Deoxyribonucleic acid; Disease; DNA; Drug dosages; Firmicutes; Flora; Gastrointestinal agents; Gut microbiota; Health aspects; Herbal medicine; Intestinal microflora; Intestine; Lactobacillus; Medicine, Chinese; Microbiota; Microbiota (Symbiotic organisms); Pathogenesis; Pharmacology, Experimental; Relative abundance; rRNA 16S; Software; Statistical analysis; Technology assessment; Traditional Chinese medicine; China
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-020-01473-3

In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.